非洲爪蟾核糖体蛋白L1在剪接水平上自身调节的结构基础。

Structural basis for autogenous regulation of Xenopus laevis ribosomal protein L1 synthesis at the splicing level.

作者信息

Gultyaev A P, Shestopalov B V

机构信息

All-Union Scientific Research Institute of Genetics, Leningrad, USSR.

出版信息

FEBS Lett. 1988 May 9;232(1):9-11. doi: 10.1016/0014-5793(88)80376-4.

DOI:10.1016/0014-5793(88)80376-4

PMID:3366251

Abstract

It is known that the injection of the Xenopus laevis ribosomal protein L1 gene into oocytes causes the accumulation of immature L1 transcripts due to a specific block of splicing of the second and third introns. In this paper the secondary structures of these introns in pre-mRNA have been constructed. It has been shown that they share homology with 28 S rRNA. The putative RNA-binding segment of L1 has also been predicted. These results are interpreted as the structural basis for autogenous regulation of X. laevis ribosomal protein L1 synthesis at the splicing level.

摘要

已知将非洲爪蟾核糖体蛋白L1基因注入卵母细胞会导致未成熟L1转录本的积累，这是由于第二和第三内含子的剪接受特定阻断所致。在本文中，已构建了前体mRNA中这些内含子的二级结构。结果表明它们与28S rRNA具有同源性。还预测了L1的假定RNA结合区段。这些结果被解释为非洲爪蟾核糖体蛋白L1合成在剪接水平上进行自体调节的结构基础。