Wormington W M
Proc Natl Acad Sci U S A. 1986 Nov;83(22):8639-43. doi: 10.1073/pnas.83.22.8639.
The synthesis of an endogenous ribosomal protein, L1, is selectively and efficiently inhibited by microinjection of antisense L1 RNAs into Xenopus oocytes. Repression of L1 synthesis is achieved within 12 hr and is maintained for 48 hr. RNase-protection assays reveal the formation of RNA X RNA duplexes in vivo between the endogenous L1 mRNA and injected antisense transcripts. Partial-length antisense RNAs, complementary to only the 3'-terminal region of L1 mRNA, repress translation as effectively as a full-length antisense RNA, indicating that complementarity to the 5' region of L1 mRNA is not required for efficient inhibition. The use of antisense RNA to repress synthesis of an endogenous ribosomal protein provides a functional basis for determining mechanisms that integrate ribosomal protein synthesis with ribosome assembly during oogenesis.
通过向非洲爪蟾卵母细胞显微注射反义L1 RNA，内源性核糖体蛋白L1的合成受到选择性且高效的抑制。L1合成的抑制在12小时内实现，并维持48小时。核糖核酸酶保护试验揭示了体内内源性L1 mRNA与注射的反义转录本之间形成RNA×RNA双链体。仅与L1 mRNA 3'末端区域互补的部分长度反义RNA，与全长反义RNA一样有效地抑制翻译，这表明有效抑制并不需要与L1 mRNA的5'区域互补。利用反义RNA抑制内源性核糖体蛋白的合成，为确定在卵子发生过程中整合核糖体蛋白合成与核糖体组装的机制提供了功能基础。
