Brouard C, Siess M H, Vernevaut M F, Suschetet M
Station de Recherches sur la Qualité des Aliments de l'Homme, Institut National de la Recherche Agronomique, Dijon, France.
Food Chem Toxicol. 1988 Feb;26(2):99-103. doi: 10.1016/0278-6915(88)90105-6.
The influence of dietary flavone and quercetin on the components of the drug metabolizing enzyme system was examined in the liver and small intestine of the rat. Quercetin given at a concentration of 1% in the diet for 14 days produced no significant changes on phase I or phase II enzyme activities. In contrast, 0.25% flavone caused significant increases in relative liver weight, microsomal and cytoplasmic proteins, and cytochrome P-450 content. The activities of hepatic ethoxyresorufin, pentoxyresorufin and ethoxycoumarin deethylases were significantly increased (by 20, 30 and 2.5-fold, respectively) over control levels. Hepatic UDP-glucuronyl transferase and glutathione transferase activities were increased 3-4-fold. In contrast, flavone induced no changes in these two intestinal enzyme activities. It is concluded that flavone produces an induction that shows both phenobarbital- and methylcholanthrene-type characteristics and that its effects on conjugating enzymes is tissue specific.
研究了膳食黄酮和槲皮素对大鼠肝脏和小肠中药物代谢酶系统各组分的影响。在饮食中添加1%的槲皮素并持续14天，对Ⅰ相或Ⅱ相酶活性未产生显著变化。相比之下，0.25%的黄酮可使肝脏相对重量、微粒体和细胞质蛋白以及细胞色素P-450含量显著增加。肝脏乙氧基试卤灵、戊氧基试卤灵和乙氧基香豆素脱乙基酶的活性比对照水平显著增加（分别增加20倍、30倍和2.5倍）。肝脏UDP-葡萄糖醛酸基转移酶和谷胱甘肽转移酶活性增加了3 - 4倍。相比之下，黄酮对这两种肠道酶活性未产生影响。得出的结论是，黄酮产生的诱导作用兼具苯巴比妥型和甲基胆蒽型特征，并且其对结合酶的影响具有组织特异性。
