Qian Changlin, Qiu Weiqing, Zhang Jie, Shen Zhiyong, Liu Hua, Zhang Yongjie
The Second Department of Biliary Surgery, Eastern Hepatobiliary Surgery Hospital, The Second Military Medical University, Shanghai, China.
Department of General Surgery, South Campus, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
PeerJ. 2021 Feb 23;9:e10803. doi: 10.7717/peerj.10803. eCollection 2021.
Cholesterol gallstone (CG) is the most common gallstone disease, which is induced by biliary cholesterol supersaturation. The purpose of this study is to investigate the pathogenesis of CG.
Sixteen mice were equally and randomly divided into model group and normal control group. The model group was fed with lithogenic diets to induce CG, and then gallbladder bile lipid analysis was performed. After RNA-seq library was constructed, differentially expressed mRNAs (DE-mRNAs) and differentially expressed lncRNAs (DE-lncRNAs) between model group and normal control group were analyzed by DESeq2 package. Using the cluster Profiler package, enrichment analysis for the DE-mRNAs was carried out. Based on Cytoscape software, the protein-protein interaction (PPI) network and competing endogenous RNA (ceRNA) network were built. Using quantitative real-time reverse transcription-PCR (qRT-PCR) analysis, the key RNAs were validated.
The mouse model of CG was suc cessfully established, and then 181 DE-mRNAs and 33 DE-lncRNAs between model and normal groups were obtained. Moreover, KDM4A was selected as a hub node in the PPI network, and lncRNA was considered as a key lncRNA in the regulatory network. Additionally, the regulatory pairs and co-expression pairs existed in the regulatory network. The qRT-PCR analysis showed that expression was increased, and the expressions of , , , and were downregulated.
These RNAs might be related to the pathogenesis of CG.
胆固醇性胆结石（CG）是最常见的胆结石疾病，由胆汁胆固醇过饱和引起。本研究旨在探讨CG的发病机制。
将16只小鼠等分为模型组和正常对照组。模型组给予致石饮食以诱导CG，然后进行胆囊胆汁脂质分析。构建RNA-seq文库后，使用DESeq2软件包分析模型组和正常对照组之间差异表达的mRNA（DE-mRNA）和差异表达的lncRNA（DE-lncRNA）。使用cluster Profiler软件包对DE-mRNA进行富集分析。基于Cytoscape软件构建蛋白质-蛋白质相互作用（PPI）网络和竞争性内源RNA（ceRNA）网络。使用定量实时逆转录PCR（qRT-PCR）分析对关键RNA进行验证。
成功建立了CG小鼠模型，模型组和正常组之间获得了181个DE-mRNA和33个DE-lncRNA。此外，KDM4A被选为PPI网络中的枢纽节点，lncRNA被认为是调控网络中的关键lncRNA。此外，调控网络中存在调控对和共表达对。qRT-PCR分析表明， 表达增加， 、 、 和 的表达下调。
这些RNA可能与CG的发病机制有关。
