鲢鱼（Hypophthalmichthys molitrix）垂体促性腺激素的分离与特性

Isolation and properties of the pituitary gonadotropin from silver carp (Hypophthalmichthys molitrix).

作者信息

Chang Y S, Huang F L, Chen C T, Lo T B

机构信息

Institute of Biological Chemistry, Academia Sinica, National Taiwan University, Taipei, R.O.C.

出版信息

Int J Pept Protein Res. 1988 Feb;31(2):150-6. doi: 10.1111/j.1399-3011.1988.tb00017.x.

DOI:10.1111/j.1399-3011.1988.tb00017.x

PMID:3366548

Abstract

The gonadotropin of silver carp (scGTH) was purified from the pituitary glands in a yield of 8.4 mg/g through extraction with 6% ammonium acetate (pH 5.1)-40% ethanol followed by 80% ethanol precipitation, DEAE-cellulose chromatography and gel filtration. The molecular weight of scGTH is about 32,000 as estimated by gel filtration. Leucine and serine were the two N-termini of scGTH. The amino acid composition of scGTH is rich in aspartic acid/asparagine, glutamic acid/glutamine, valine, proline and half cystine. Its carbohydrate content is 22.2%. Biologically, scGTH significantly stimulated the steroidogenesis in carp testis and ovary in vitro and induced the ovulation in loaches. It also stimulated the 32P-phosphate uptake into 1-day-old chicken testis. Immunologically, scGTH could cross-react with the antiserum against cGTH and vice versa. In both cases parallel displacement curves were displayed.

摘要

从鲢鱼脑垂体中纯化出鲢鱼促性腺激素（scGTH），通过用6%醋酸铵（pH 5.1）-40%乙醇提取，随后进行80%乙醇沉淀、DEAE-纤维素层析和凝胶过滤，产率为8.4 mg/g。通过凝胶过滤估计，scGTH的分子量约为32,000。亮氨酸和丝氨酸是scGTH的两个N端。scGTH的氨基酸组成富含天冬氨酸/天冬酰胺、谷氨酸/谷氨酰胺、缬氨酸、脯氨酸和半胱氨酸。其碳水化合物含量为22.2%。在生物学上，scGTH在体外显著刺激鲤鱼睾丸和卵巢的类固醇生成，并诱导泥鳅排卵。它还刺激32P-磷酸盐进入1日龄鸡睾丸。在免疫学上，scGTH能与抗鲤鱼促性腺激素（cGTH）抗血清发生交叉反应，反之亦然。在这两种情况下都呈现出平行的置换曲线。