Winkler B S, Solomon F
Eye Research Institute of Oakland University, Rochester, MI 48309.
Invest Ophthalmol Vis Sci. 1988 May;29(5):821-3.
Measurements were made of the activities of the NADP+-dependent isocitrate dehydrogenase, malic enzyme and glucose-6-phosphate dehydrogenase in cytosolic supernatants of whole lens and capsule-epithelium. The activities of all three NADP+-dependent enzymes were concentrated in the capsule-epithelium relative to the activities measured in the whole lens. These results show for the first time that the rabbit lens epithelial cells contain substantial activities of the NADP+-dependent isocitrate dehydrogenase and malic enzyme. It is proposed that these enzymes together with glucose-6-phosphate dehydrogenase are involved in the regulation of the intracellular level of glutathione during oxidative stress by providing the NADPH necessary for the activity of glutathione reductase.
对整个晶状体和晶状体囊上皮细胞胞质上清液中依赖NADP⁺的异柠檬酸脱氢酶、苹果酸酶和葡萄糖-6-磷酸脱氢酶的活性进行了测定。相对于在整个晶状体中测得的活性，这三种依赖NADP⁺的酶的活性都集中在晶状体囊上皮细胞中。这些结果首次表明，兔晶状体上皮细胞含有大量依赖NADP⁺的异柠檬酸脱氢酶和苹果酸酶活性。有人提出，这些酶与葡萄糖-6-磷酸脱氢酶一起，通过提供谷胱甘肽还原酶活性所需的NADPH，参与氧化应激期间细胞内谷胱甘肽水平的调节。
