Welsch U, Buchheim W, Schumacher U, Schinko I, Patton S
Anatomische Anstalt, Lehrstuhl II, Ludwig-Maximilians-Universität, München, Federal Republic of Germany.
Histochemistry. 1988;88(3-6):357-65. doi: 10.1007/BF00570295.
Horse milk fat globules (MFGs) and casein micelles were studied using freeze fracturing, freeze etching and thin-section electron microscopy, as well as lectin histochemistry, gel electrophoresis, and Western blotting. Horse MFGs were found to be relatively small, their average volume-surface diameter being about 2.75 microns. The MFG membrane is composed of three layers: an inner proteinaceous coat occasionally having a paracrystalline substructure, a unit membrane, and a prominent filamentous glycocalyx. The last is rich in glycoconjugates, as revealed by its binding of various lectins. In addition, the glycocalyx binds cationized ferritin, which indicates the presence of negative electric charges. Gel electrophoresis revealed the presence of high-molecular-weight glycoproteins in the MFG membrane of horse milk. Such glycoproteins are also present in human MFG membranes but are absent in the bovine MFGs. The casein micelles in horse milk are relatively large, their average volume-surface diameter being about 200 nm.
利用冷冻断裂、冷冻蚀刻和超薄切片电子显微镜技术，以及凝集素组织化学、凝胶电泳和蛋白质印迹法对马乳脂肪球（MFGs）和酪蛋白胶粒进行了研究。发现马的MFGs相对较小，其平均体积-表面直径约为2.75微米。MFG膜由三层组成：内层蛋白质 coat 偶尔具有准晶亚结构、单位膜和突出的丝状糖萼。如通过其与各种凝集素的结合所揭示的，最后一层富含糖缀合物。此外，糖萼结合阳离子化铁蛋白，这表明存在负电荷。凝胶电泳显示马乳MFG膜中存在高分子量糖蛋白。此类糖蛋白也存在于人类MFG膜中，但在牛的MFGs中不存在。马乳中的酪蛋白胶粒相对较大，其平均体积-表面直径约为200纳米。
