Institute of Ecology and Genetics of Microorganisms, Ural Branch of the Russian Academy of Sciences - Filial of the Perm Federal Research Centre of the Ural Branch of the Russian Academy of Sciences, 13 Golev Street, Perm 614081, Russia.
Institute of Technical Chemistry, Filial of the Perm Federal Research Centre of the Ural Branch of the Russian Academy of Sciences, 3 Akademika Koroleva Street, Perm 614013, Russia.
Can J Microbiol. 2021 Apr;67(4):342-348. doi: 10.1139/cjm-2020-0299. Epub 2021 Mar 5.
The available information on synthesized compatible solutes in response to high medium salinity by bacteria of the genus is limited to studies of the mesophilic moderately halophilic strain DSM 3043. Therefore, there is a need for studies of representatives of other species of the genus of the family. A moderately halophilic psychrotolerant bacterium, strain N1, closely related to the species was isolated from the salt crust of a rock salt waste pile in Berezniki, Perm Krai, Russia. An intracellular pool of compatible solutes of strain N1 was investigated by NMR spectroscopy. Cells grown in the presence of 5% NaCl at optimal growth temperature (28 °C) accumulated ectoine, glutamate, (4)-acetyl-l-2,4-diaminobutyrate (NADA), alanine, trehalose, hydroxyectoine, and valine. Such a combination of compatible solutes is unique and distinguishes the strain from DSM 3043. Hyperosmotic stress induced by 15% NaCl caused the accumulation of ectoine, NADA, and hydroxyectoine but led to a decrease in the amount of alanine, valine, and trehalose. The intracellular pool of glutamate was not significantly changed. A reduction of the growth temperature from 28 to 5 °C led to an increase in the amount of ectoine, NADA, trehalose, and hydroxyectoine. Ectoine was the major compatible solute.
关于该属细菌对高介质盐度的合成相容溶质的可用信息仅限于对嗜温中度嗜盐菌株 DSM 3043 的研究。因此，需要对该科的其他属种的代表进行研究。一种中度嗜盐耐冷细菌，菌株 N1，与物种密切相关，从俄罗斯彼尔姆边疆区 Berezniki 的岩盐废物堆的盐壳中分离出来。通过 NMR 光谱研究了菌株 N1 的细胞内相容溶质库。在最佳生长温度（28°C）下，用 5%NaCl 培养的细胞积累了海藻糖、谷氨酸、（4）-乙酰-l-2,4-二氨基丁酸（NADA）、丙氨酸、海藻糖、羟基海藻糖和缬氨酸。这种相容溶质的组合是独特的，将该菌株与 DSM 3043 区分开来。15%NaCl 诱导的高渗胁迫导致积累了海藻糖、NADA 和羟基海藻糖，但导致丙氨酸、缬氨酸和海藻糖的量减少。谷氨酸的细胞内库没有明显变化。生长温度从 28°C 降低到 5°C 导致海藻糖、NADA、海藻糖和羟基海藻糖的量增加。海藻糖是主要的相容溶质。
