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宜昌橙气味活性成分的表征及其DNA基因分型法的种间遗传起源鉴定

Characterization of Odor-Active Compounds of Ichang Lemon () and Identification of Its Genetic Interspecific Origin by DNA Genotyping.

作者信息

Demarcq Benoit, Cavailles Margaux, Lambert Laetitia, Schippa Christine, Ollitrault Patrick, Luro Francois

机构信息

V Mane Fils SA, 620 Route de Grasse, 06620 Le Bar-sur-Loup, France.

CIRAD, UMR AGAP, F-20230 San Giuliano, France.

出版信息

J Agric Food Chem. 2021 Mar 17;69(10):3175-3188. doi: 10.1021/acs.jafc.0c07894. Epub 2021 Mar 5.

DOI:10.1021/acs.jafc.0c07894
PMID:33667086
Abstract

Ichang lemon is a citrus fruit whose rind gives off a delicious and much appreciated fragrance and flavor. The volatile components of the fruit peel of Ichang lemon were investigated by GC-MS and GC-O (AEDA method). Simultaneously, its genetic origin was identified by using diagnostic SNP markers specific to ancestral species and multiallelic SSR and InDel markers. Ichang lemon combines three ancestral genomes (, , and ) and may be a pummelo × Yuzu hybrid. Although the major compounds of the Ichang lemon aromatic profile were present in , a few pummelo-specific compounds were also detected, such as indole and nootkatone, in agreement with its maternal lineage. 3-Methyl-3-sulfanylbutyl acetate, reported to occur in passion fruit and brewed coffee, was identified by GC-MS, GC-QTOF-MS, and GC-FTIR for the first time in citrus. This odor-active compound has a sulfurous, tropical fruity, green note.

摘要

宜昌橙是一种柑橘类水果,其果皮散发出美味且备受赞赏的香气和风味。采用气相色谱 - 质谱联用(GC - MS)和气相色谱 - 嗅闻(GC - O,香气提取物稀释分析方法)对宜昌橙果皮的挥发性成分进行了研究。同时,利用特定于祖先物种的诊断性单核苷酸多态性(SNP)标记以及多等位基因简单重复序列(SSR)和插入缺失(InDel)标记确定了其遗传起源。宜昌橙结合了三个祖先基因组(、和),可能是柚与枸橼的杂交种。尽管宜昌橙香气特征的主要成分在中也有,但也检测到了一些柚特有的化合物,如吲哚和诺卡酮,这与其母系谱系一致。乙酸3 - 甲基 - 3 - 硫代丁酯,据报道存在于西番莲果和煮咖啡中,首次通过气相色谱 - 质谱联用(GC - MS)、气相色谱 - 四极杆飞行时间质谱联用(GC - QTOF - MS)和气相色谱 - 傅里叶变换红外光谱联用(GC - FTIR)在柑橘类水果中被鉴定出来。这种气味活性化合物具有硫磺味、热带水果味和青香调。

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