Centro de Edafología y Biología Aplicada del Segura (CEBAS-CSIC), Departamento de Biología del Estrés y Patología Vegetal, Grupo de Patología Vegetal, 30100 Murcia, Spain.
Abiopep S.L., R&D Department, Parque Científico de Murcia, Ctra. de Madrid, Km 388, Complejo de Espinardo, Edf. R, 2º, 30100 Murcia, Spain.
Viruses. 2021 Feb 25;13(3):358. doi: 10.3390/v13030358.
Plant viruses are obligate parasites that need to usurp plant cell metabolism in order to infect their hosts. Imaging techniques have been used for quite a long time to study plant virus-host interactions, making it possible to have major advances in the knowledge of plant virus infection cycles. The imaging techniques used to study plant-virus interactions have included light microscopy, confocal laser scanning microscopy, and scanning and transmission electron microscopies. Here, we review the use of these techniques in plant virology, illustrating recent advances in the area with examples from plant virus replication and virus plant-to-plant vertical transmission processes.
植物病毒是专性寄生物,为了感染宿主,它们需要篡夺植物细胞的新陈代谢。成像技术已经被用于研究植物-病毒相互作用相当长的一段时间,使得在植物病毒感染周期的知识方面取得了重大进展。用于研究植物-病毒相互作用的成像技术包括光学显微镜、共聚焦激光扫描显微镜以及扫描和透射电子显微镜。在这里,我们回顾了这些技术在植物病毒学中的应用,并用植物病毒复制和病毒植物-植物垂直传播过程的例子来说明该领域的最新进展。