Canalis E, Centrella M, McCarthy T
Department of Medicine (Endocrine Section), Saint Francis Hospital and Medical Center, Hartford, Connecticut 06105.
J Clin Invest. 1988 May;81(5):1572-7. doi: 10.1172/JCI113490.
Basic fibroblast growth factor (bFGF) was studied for its effects on bone formation in cultured rat calvariae. bFGF at 0.1-100 ng/ml stimulated [3H]thymidine incorporation into DNA by up to 4.4-fold. bFGF also increased the number of colcemid-induced metaphase arrested cells and the DNA content. Transient (24 h) treatment with bFGF enhanced [3H]-proline incorporation into collagen 24-48 h after the factor was removed; this effect was DNA synthesis dependent and blocked by hydroxyurea. The collagen stimulated by bFGF was type I, and this effect was observed primarily in the periosteum-free bone. In contrast, continuous treatment with bFGF for 24-96 h inhibited [3H]proline incorporation into type I collagen. bFGF did not alter collagen degradation. In conclusion, bFGF stimulates calvarial DNA synthesis, which causes an increased number of collagen-synthesizing cells, but bFGF has a direct inhibitory effect on collagen synthesis.
研究了碱性成纤维细胞生长因子（bFGF）对培养的大鼠颅骨骨形成的影响。0.1-100 ng/ml的bFGF可使[3H]胸苷掺入DNA的量增加达4.4倍。bFGF还增加了秋水仙酰胺诱导的中期停滞细胞的数量和DNA含量。用bFGF进行短暂（24小时）处理后，在去除该因子24-48小时后可增强[3H] - 脯氨酸掺入胶原蛋白的能力；这种效应依赖于DNA合成，并被羟基脲阻断。bFGF刺激产生的胶原蛋白为I型，且这种效应主要在无骨膜的骨中观察到。相反，用bFGF连续处理24-96小时会抑制[3H]脯氨酸掺入I型胶原蛋白。bFGF不会改变胶原蛋白的降解。总之，bFGF刺激颅骨DNA合成，导致胶原蛋白合成细胞数量增加，但bFGF对胶原蛋白合成有直接抑制作用。
Zotero 插件