Shoji Shintaro, Uchida Kentaro, Saito Wataru, Sekiguchi Hiroyuki, Inoue Gen, Miyagi Masayuki, Kuroda Akiyoshi, Takaso Masashi
Orthopaedic Surgery, Kitasato University School of Medicine, Sagamihara, JPN.
Orthopaedics, Shonan University, Chigasaki, JPN.
Cureus. 2020 Aug 27;12(8):e10085. doi: 10.7759/cureus.10085.
An enzymatic crosslinking strategy using hydrogen peroxide (HO) and horseradish peroxidase (HRP) has been receiving increasing attention for use with in situ-formed hydrogels (IFHs). Several studies have reported the application of IFHs in cell delivery and tissue engineering. IFHs may also be ideal carrier materials for bone repair, although their potential as a carrier for basic fibroblast growth factor (bFGF) has yet to be evaluated. Here, we examined the effect of an IFH made of dextran (Dex)-tyramine (TA) conjugates (IFH-Dex-TA) containing bFGF in promoting bone formation in a fracture model in mice. Immediately following a fracture procedure, animals either received no treatment (control) or an injection of IFH-Dex-TA/phosphate-buffered saline (IFH-Dex-TA/PBS) or IFH-Dex-TA containing 1 μg bFGF (IFH-Dex-TA/bFGF) into the fracture site (n=10, each treatment). Fracture sites injected with IFH-Dex-TA/bFGF showed significantly greater bone volume, mineral content, and bone union than sites receiving no treatment or treated with IFH-Dex-TA/PBS alone (each n=10). This Dex-TA gel may be an effective drug delivery system for optimizing bFGF therapy.
一种使用过氧化氢（HO）和辣根过氧化物酶（HRP）的酶促交联策略，在原位形成水凝胶（IFH）的应用中受到越来越多的关注。几项研究报道了IFH在细胞递送和组织工程中的应用。IFH也可能是骨修复的理想载体材料，尽管其作为碱性成纤维细胞生长因子（bFGF）载体的潜力尚未得到评估。在此，我们研究了含有bFGF的葡聚糖（Dex）-酪胺（TA）缀合物制成的IFH（IFH-Dex-TA）在促进小鼠骨折模型骨形成中的作用。骨折手术后，动物要么不接受治疗（对照组），要么在骨折部位注射IFH-Dex-TA/磷酸盐缓冲盐水（IFH-Dex-TA/PBS）或含有1μg bFGF的IFH-Dex-TA（IFH-Dex-TA/bFGF）（每组治疗n=10）。注射IFH-Dex-TA/bFGF的骨折部位比未接受治疗或仅用IFH-Dex-TA/PBS治疗的部位显示出明显更大的骨体积、矿物质含量和骨愈合（每组n=10）。这种Dex-TA凝胶可能是优化bFGF治疗的有效药物递送系统。
