Norena-Caro Daniel A, Malone Tara M, Benton Michael G
Cain Department of Chemical Engineering, 3307 Patrick F. Taylor Hall, Louisiana State University and A&M College, Baton Rouge, LA 70803, USA.
Microorganisms. 2021 Feb 19;9(2):431. doi: 10.3390/microorganisms9020431.
sp. UTEX 2576 metabolizes multiple nitrogen (N) sources and is deemed a biotechnological platform for chemical production. Cyanobacteria have been identified as prolific producers of biofertilizers, biopolymers, biofuels, and other bioactive compounds. Here, we analyze the effect of different N-sources and Fe availability on the bioproduction of phycobiliproteins and β-carotene. We characterize nutrient demand in modified BG11 media, including data on CO fixation rates, N-source consumption, and mineral utilization (e.g., phosphorus (P), and 11 metallic elements). Results suggest that non-diazotrophic cultures grow up to 60% faster than diazotrophic cells, resulting in 20% higher CO-fixation rates. While the production of β-carotene was maximum in medium with NaNO, Fe starvation increased the cellular abundance of C-phycocyanin and allophycocyanin by at least 22%. Compared to cells metabolizing NaNO and N, cultures adapted to urea media increased their P, calcium and manganese demands by at least 72%, 97% and 76%, respectively. Variations on pigmentation and nutrient uptake were attributed to changes in phycocyanobilin biosynthesis, light-induced oxidation of carotenoids, and urea-promoted peroxidation. This work presents insights into developing optimal culture for efficient operations of bioproduction and wastewater bioremediation with cyanobacteria.
UTEX 2576菌株能够代谢多种氮源，被视为用于化学品生产的生物技术平台。蓝细菌已被确认为生物肥料、生物聚合物、生物燃料及其他生物活性化合物的丰富生产者。在此，我们分析了不同氮源和铁有效性对藻胆蛋白和β-胡萝卜素生物合成的影响。我们对改良BG11培养基中的营养需求进行了表征，包括二氧化碳固定率、氮源消耗及矿物质利用（如磷和11种金属元素）的数据。结果表明，非固氮培养物的生长速度比固氮细胞快60%，导致二氧化碳固定率提高20%。虽然在含有硝酸钠的培养基中β-胡萝卜素的产量最高，但铁饥饿使C-藻蓝蛋白和别藻蓝蛋白的细胞丰度至少增加了22%。与代谢硝酸钠和氮的细胞相比，适应尿素培养基的培养物对磷、钙和锰的需求分别至少增加了72%、97%和76%。色素沉着和营养吸收的变化归因于藻蓝胆素生物合成的改变、光诱导的类胡萝卜素氧化以及尿素促进的过氧化作用。这项工作为开发用于蓝细菌高效生物生产和废水生物修复的最佳培养条件提供了见解。
