Rybak Zbigniew, Janeczek Maciej, Dobrzynski Maciej, Wujczyk Marta, Czerski Albert, Kuropka Piotr, Noszczyk-Nowak Agnieszka, Szymonowicz Maria, Sender-Janeczek Aleksandra, Wiglusz Katarzyna, Wiglusz Rafal J
Department for Experimental Surgery and Biomaterials Research, Wroclaw Medical University, Bujwida 44, 50-345 Wroclaw, Poland.
Department of Biostructure and Animal Physiology, Wroclaw University of Environmental and Life Sciences, Kozuchowska 1, 51-631 Wroclaw, Poland.
Nanomaterials (Basel). 2021 Feb 21;11(2):544. doi: 10.3390/nano11020544.
Sclerotherapy is the chemical occlusion of vessels using an intravenous injection of a liquid or foamed sclerosing agent that is used in the therapy of blood and lymphatic vessels malformations in the young, and for spider veins, smaller varicose veins, hemorrhoids and hydroceles in adults. This study aimed to assess the effectiveness of mechanosclerotherapy of venous veins with a new device-Flebogrif-based on an animal model. The experiment was performed on nine Polish Merino sheep weighing 40-50 kilograms. The animals were anesthetized intravenously. The material was divided into three groups: two experimental (1 and 2) and control (3) group. The first experimental group was treated with the use of Flebogrif and a sclerosant simultaneously, while only Flebogrif was used in the second experimental group. Flebogrif was applied into the lateral saphenous vein of both pelvic limbs. The vessel wall thickness was estimated at four points of the histological image in mm (V1, V2, V3, V4). For one month, the animals were euthanized, and the occlusion rate of the treated veins and changes in the vein wall were determined. Histological slides were analyzed under a light microscope and histometry of the vein wall was performed. The Shapiro-Wilk test and the quantity of the investigated parameter groups allowed for using a non-parametric method at four points to compare thickness measurements (the Mann-Whitney test), with < 0.05. The Mann-Whitney test indicated statistically significant differences between both experimental groups. The results obtained from morphometrical and histological analysis showed better results in the first experimental group than those of the second experimental group. Finally, statistical analysis revealed significant differences between the both the experimental group and control group in morphological analysis. The achieved results allowed us to conclude that the simultaneous use of Flebogrif and a sclerosant yielded better results of vein lumen reduction than the use of Flebogrif alone.
硬化疗法是通过静脉注射液体或泡沫硬化剂来化学闭塞血管，该方法用于治疗青少年的血液和淋巴管畸形，以及成人的蜘蛛状静脉、较小的静脉曲张、痔疮和鞘膜积液。本研究旨在基于动物模型评估一种新型设备——Flebogrif进行静脉机械硬化疗法的有效性。实验在9只体重40 - 50千克的波兰美利奴绵羊身上进行。动物通过静脉注射麻醉。材料分为三组：两个实验组（1组和2组）和对照组（3组）。第一实验组同时使用Flebogrif和硬化剂进行治疗，而第二实验组仅使用Flebogrif。将Flebogrif应用于双侧后肢的大隐静脉。在组织学图像的四个点（V1、V2、V3、V4）处估计血管壁厚度，单位为毫米。一个月后，对动物实施安乐死，并确定治疗静脉的闭塞率以及静脉壁的变化。在光学显微镜下分析组织学切片，并对静脉壁进行组织测量。Shapiro-Wilk检验以及所研究参数组的数量允许在四个点使用非参数方法比较厚度测量值（Mann-Whitney检验），P < 0.05。Mann-Whitney检验表明两个实验组之间存在统计学显著差异。形态学和组织学分析结果显示，第一实验组的结果优于第二实验组。最后，统计分析表明在形态学分析中实验组和对照组之间存在显著差异。所取得的结果使我们得出结论，与单独使用Flebogrif相比，同时使用Flebogrif和硬化剂在减少静脉管腔方面产生了更好的效果。
