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市售环介导等温扩增(LAMP)试剂盒在疟疾流行和非流行地区的性能及应用

Performance and Application of Commercially Available Loop-Mediated Isothermal Amplification (LAMP) Kits in Malaria Endemic and Non-Endemic Settings.

作者信息

Morris Ulrika, Aydin-Schmidt Berit

机构信息

Department of Microbiology, Tumor, and Cell Biology, Karolinska Institutet, 17177 Stockholm, Sweden.

出版信息

Diagnostics (Basel). 2021 Feb 18;11(2):336. doi: 10.3390/diagnostics11020336.


DOI:10.3390/diagnostics11020336
PMID:33670618
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7922894/
Abstract

Loop-mediated isothermal amplification (LAMP) is a sensitive molecular tool suitable for use as a near point-of-care test for the diagnosis of malaria. Recent meta-analyses have detailed high sensitivity and specificity of malaria LAMP when compared to microscopy, rapid diagnostic tests, and polymerase chain reaction in both endemic and non-endemic settings. Despite this, the use of malaria LAMP has primarily been limited to research settings to date. In this review, we aim to assess to what extent commercially available malaria LAMP kits have been applied in different settings, and to identify possible obstacles that may have hindered their use from being adopted further. In order to address this, we conducted a literature search in PubMed.gov using the search terms (((LAMP) OR (Loop-mediated isothermal amplification)) AND ((Malaria) OR (Plasmodium))). Focusing primarily on studies employing one of the commercially available kits, we then selected three key areas of LAMP application for further review: the performance and application of LAMP in malaria endemic settings including low transmission areas; LAMP for malaria screening during pregnancy; and malaria LAMP in returning travelers in non-endemic settings.

摘要

环介导等温扩增技术(LAMP)是一种灵敏的分子检测工具,适用于疟疾诊断的即时检测。最近的荟萃分析详细说明了在疟疾流行地区和非流行地区,与显微镜检查、快速诊断检测及聚合酶链反应相比,疟疾LAMP检测具有高灵敏度和特异性。尽管如此,到目前为止,疟疾LAMP检测的应用主要局限于研究领域。在本综述中,我们旨在评估市售疟疾LAMP试剂盒在不同环境中的应用程度,并确定可能阻碍其进一步推广使用的潜在障碍。为解决这一问题,我们在PubMed.gov上进行了文献检索,检索词为(((LAMP)或(环介导等温扩增技术))且((疟疾)或(疟原虫)))。我们主要关注使用市售试剂盒之一的研究,然后选择了LAMP应用的三个关键领域进行进一步综述:LAMP在疟疾流行地区(包括低传播地区)的性能和应用;孕期疟疾筛查的LAMP检测;以及非流行地区归国旅行者的疟疾LAMP检测。

相似文献

[1]
Performance and Application of Commercially Available Loop-Mediated Isothermal Amplification (LAMP) Kits in Malaria Endemic and Non-Endemic Settings.

Diagnostics (Basel). 2021-2-18

[2]
Diagnostic accuracy of loop-mediated isothermal amplification (LAMP) for screening malaria in peripheral and placental blood samples from pregnant women in Colombia.

Malar J. 2018-7-13

[3]
Expanding the malaria molecular diagnostic options: opportunities and challenges for loop-mediated isothermal amplification tests for malaria control and elimination.

Expert Rev Mol Diagn. 2018-1-28

[4]
Loop-mediated isothermal amplification (LAMP) assay for the diagnosis of imported malaria: a narrative review.

Infez Med. 2021-9-10

[5]
Diagnostic accuracy of loop-mediated isothermal amplification (LAMP) for screening patients with imported malaria in a non-endemic setting.

Parasite. 2017

[6]
Diagnostic performance of the loop-mediated isothermal amplification (LAMP) based illumigene malaria assay in a non-endemic region.

Malar J. 2017-10-17

[7]
Loop-mediated isothermal amplification (LAMP) test for diagnosis of uncomplicated malaria in endemic areas: a meta-analysis of diagnostic test accuracy.

Malar J. 2020-6-19

[8]
Clinical accuracy of malaria loop-mediated isothermal amplification assay as a stand-alone screening tool at a non-endemic Northern California regional health system.

Diagn Microbiol Infect Dis. 2022-6

[9]
Performance evaluation of different strategies based on microscopy techniques, rapid diagnostic test and molecular loop-mediated isothermal amplification assay for the diagnosis of imported malaria.

Clin Microbiol Infect. 2019-5-31

[10]
Evaluation of the loop mediated isothermal DNA amplification (LAMP) kit for malaria diagnosis in P. vivax endemic settings of Colombia.

PLoS Negl Trop Dis. 2015-1-8

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[2]
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Curr Med Mycol. 2024-11-14

[3]
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Malar J. 2025-4-23

[4]
Unveiling the silent threat: Investigating asymptomatic plasmodium infections in Gorgora, Ethiopia through microscopy and loop-mediated isothermal amplification.

PLoS One. 2025-3-6

[5]
Development and Validation of a Highly Sensitive RT-qLAMP Assay for Rapid Detection of SARS-CoV-2: Methodological Aspects.

Mol Biotechnol. 2024-9-24

[6]
Circulation of Non- Species in Niger: Implications for Malaria Diagnosis.

Open Forum Infect Dis. 2024-8-19

[7]
Head-to-head comparison of two loop-mediated isothermal amplification (LAMP) kits for diagnosis of malaria in a non-endemic setting.

Malar J. 2023-12-13

[8]
Genetic diversity of among immigrant patients exhibiting severe and non-severe clinical manifestations in northern suburbs of Paris.

Epidemiol Infect. 2023-10-2

[9]
Molecular detection of human Plasmodium species using a multiplex real time PCR.

Sci Rep. 2023-7-14

[10]
Malaria diagnosis using a combined system of a simple and fast extraction method with a lyophilised Dual-LAMP assay in a non-endemic setting.

Pathog Glob Health. 2024-2

本文引用的文献

[1]
Ultrasensitive Diagnostics for Low-Density Asymptomatic Plasmodium falciparum Infections in Low-Transmission Settings.

J Clin Microbiol. 2021-3-19

[2]
Why Asymptomatic Plasmodium falciparum Infections Are Common in Low-Transmission Settings.

Trends Parasitol. 2020-8-25

[3]
Active case detection of malaria in pregnancy using loop-mediated amplification (LAMP): a pilot outcomes study in South West Ethiopia.

Malar J. 2020-8-27

[4]
Diagnosis of by Loop-Mediated Isothermal Amplification in Febrile Patient Samples from Loreto, Perú.

Am J Trop Med Hyg. 2020-10

[5]
Evaluation of highly sensitive diagnostic tools for the detection of P. falciparum in pregnant women attending antenatal care visits in Colombia.

BMC Pregnancy Childbirth. 2020-7-31

[6]
Systematic review and meta-analysis of diagnostic accuracy of loop-mediated isothermal amplification (LAMP) methods compared with microscopy, polymerase chain reaction and rapid diagnostic tests for malaria diagnosis.

Int J Infect Dis. 2020-7-11

[7]
The impact of gravidity, symptomatology and timing of infection on placental malaria.

Malar J. 2020-6-24

[8]
Loop-mediated isothermal amplification (LAMP) test for diagnosis of uncomplicated malaria in endemic areas: a meta-analysis of diagnostic test accuracy.

Malar J. 2020-6-19

[9]
Relationships Between Measures of Malaria at Delivery and Adverse Birth Outcomes in a High-Transmission Area of Uganda.

J Infect Dis. 2020-8-4

[10]
Asymptomatic Submicroscopic Infection Is Highly Prevalent and Is Associated with Anemia in Children Younger than 5 Years in South Kivu/Democratic Republic of Congo.

Am J Trop Med Hyg. 2020-5

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