Morris Ulrika, Aydin-Schmidt Berit
Department of Microbiology, Tumor, and Cell Biology, Karolinska Institutet, 17177 Stockholm, Sweden.
Diagnostics (Basel). 2021 Feb 18;11(2):336. doi: 10.3390/diagnostics11020336.
Loop-mediated isothermal amplification (LAMP) is a sensitive molecular tool suitable for use as a near point-of-care test for the diagnosis of malaria. Recent meta-analyses have detailed high sensitivity and specificity of malaria LAMP when compared to microscopy, rapid diagnostic tests, and polymerase chain reaction in both endemic and non-endemic settings. Despite this, the use of malaria LAMP has primarily been limited to research settings to date. In this review, we aim to assess to what extent commercially available malaria LAMP kits have been applied in different settings, and to identify possible obstacles that may have hindered their use from being adopted further. In order to address this, we conducted a literature search in PubMed.gov using the search terms (((LAMP) OR (Loop-mediated isothermal amplification)) AND ((Malaria) OR (Plasmodium))). Focusing primarily on studies employing one of the commercially available kits, we then selected three key areas of LAMP application for further review: the performance and application of LAMP in malaria endemic settings including low transmission areas; LAMP for malaria screening during pregnancy; and malaria LAMP in returning travelers in non-endemic settings.
环介导等温扩增技术(LAMP)是一种灵敏的分子检测工具,适用于疟疾诊断的即时检测。最近的荟萃分析详细说明了在疟疾流行地区和非流行地区,与显微镜检查、快速诊断检测及聚合酶链反应相比,疟疾LAMP检测具有高灵敏度和特异性。尽管如此,到目前为止,疟疾LAMP检测的应用主要局限于研究领域。在本综述中,我们旨在评估市售疟疾LAMP试剂盒在不同环境中的应用程度,并确定可能阻碍其进一步推广使用的潜在障碍。为解决这一问题,我们在PubMed.gov上进行了文献检索,检索词为(((LAMP)或(环介导等温扩增技术))且((疟疾)或(疟原虫)))。我们主要关注使用市售试剂盒之一的研究,然后选择了LAMP应用的三个关键领域进行进一步综述:LAMP在疟疾流行地区(包括低传播地区)的性能和应用;孕期疟疾筛查的LAMP检测;以及非流行地区归国旅行者的疟疾LAMP检测。
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