Fereig Ragab M, Abdelbaky Hanan H, Nishikawa Yoshifumi
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan.
Department of Animal Medicine, Faculty of Veterinary Medicine, South Valley University, Qena City, Qena 83523, Egypt.
Vaccines (Basel). 2021 Feb 15;9(2):155. doi: 10.3390/vaccines9020155.
Vaccination is the mainstay of preventative measures for numerous infectious diseases. infection induces storms of abortion in pregnant cows and ewes, resulting in drastic economic losses because of fetal losses and culling of the dams. Herein, we evaluated the potential of recombinant protein of dense granule protein 6 fused with glutathione--transferase (NcGRA6+GST) as a vaccine candidate against neosporosis in a pregnant mouse model. The protective efficacy was investigated by subcutaneous inoculation of BALB/c mice with recombinant NcGRA6+GST (25 pmol), and GST alone (25 pmol) or phosphate-buffered saline (PBS) as the controls. This study revealed the partial ability of NcGRA6+GST to protect the dams and offspring from infection during the critical period of pregnancy. This ability was revealed by higher survival rate and lower parasite burden in brains of offspring of the NcGRA6+GST-immunized group in comparison with the control groups. In addition, mouse dams from NcGRA6+GST-immunized groups exhibited lower clinical score and minimum alteration in body weight in comparison with PBS or GST groups after challenge with tachyzoites. Taken together, our results suggest the efficacy of recombinant NcGRA6 for interrupting the vertical transmission of in mice by reducing the severity of infections in dams and offspring.
疫苗接种是多种传染病预防措施的主要手段。感染会导致怀孕母牛和母羊流产风暴，由于胎儿损失和母畜被淘汰而造成巨大经济损失。在此，我们评估了与谷胱甘肽-S-转移酶融合的致密颗粒蛋白6重组蛋白（NcGRA6+GST）作为怀孕小鼠模型中抗新孢子虫病疫苗候选物的潜力。通过给BALB/c小鼠皮下接种重组NcGRA6+GST（25 pmol）、单独的GST（25 pmol）或磷酸盐缓冲盐水（PBS）作为对照，研究其保护效果。本研究揭示了NcGRA6+GST在怀孕关键期保护母畜和后代免受感染的部分能力。与对照组相比，NcGRA6+GST免疫组后代大脑中的存活率更高且寄生虫负担更低，从而揭示了这种能力。此外，与用速殖子攻击后的PBS或GST组相比，NcGRA6+GST免疫组的母鼠表现出更低的临床评分和体重最小变化。综上所述，我们的结果表明重组NcGRA6通过降低母畜和后代感染的严重程度，在小鼠中阻断新孢子虫垂直传播的有效性。
