Park Heanim, Seo Ji Won, Lee Tae Kyung, Kim Jae Hwan, Kim Jong-Eun, Lim Tae-Gyu, Park Jung Han Yoon, Huh Chul Sung, Yang Hee, Lee Ki Won
Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Korea.
Department of Food Science and Technology, Korea National University of Transportation, Jeungpyeong 27909, Korea.
Antioxidants (Basel). 2021 Feb 15;10(2):291. doi: 10.3390/antiox10020291.
Yak-Kong is a type of black soybean that is colloquially referred to as the "medicinal bean" and it elicits several beneficial effects that are relevant to human health, including attenuating the formation of skin wrinkles. It has previously been shown that soybean extracts elicit additional bioactivity that is fermented by lactic acid bacteria. In this study of lactic acid bacteria strains that were isolated from the stools of breast-feeding infants (<100 days old), we selected subsp. Lactis LDTM 8102 (LDTM 8102) as the lead strain for the fermentation of Yak-Kong. We investigated the effects of LDTM 8102-fermented Yak-Kong on solar-ultraviolet irradiation (sUV)-induced wrinkle formation. In HaCaT cells, the ethanol extract of LDTM 8102-fermented Yak-Kong (EFY) effectively reduced sUV-induced matrix metalloproteinase-1 (MMP-1) secretion. The effect of EFY was superior to that of unfermented (UFY)- and Lactis KCTC 5854 (another species)-fermented Yak-Kong. Additionally, EFY reduced sUV-induced MMP-1 mRNA expression and promoter activity, as well as the transactivation of AP-1 and phosphorylation of ERK1/2 and JNK1/2. Furthermore, EFY alleviated sUV-induced MMP-1 secretion, the destruction of the epidermis, and degradation of collagen in a three-dimensional (3D) skin culture model. EFY had a higher total polyphenol content and anti-oxidative activity than UFY. Twelve metabolites were significantly (≥2-fold) increased in Yak-Kong extract after fermentation by LDTM 8102. Among them, the metabolites of major isoflavones, such as 6,7,4'-trihydroxyisoflavone (THIF), exerted the reducing effect of MMP-1, which indicated that the isoflavone metabolites contributed to the effect of EFY on MMP-1 expression as active compounds. These findings suggest that EFY is a potent natural material that can potentially prevent sUV-induced wrinkle formation.
黑豆是一种黑豆品种，俗称“药豆”，它具有多种对人体健康有益的功效，包括减少皮肤皱纹的形成。此前的研究表明，大豆提取物具有由乳酸菌发酵产生的额外生物活性。在这项从母乳喂养婴儿（小于100天大）粪便中分离乳酸菌菌株的研究中，我们选择了乳酸乳球菌亚种LDTM 8102（LDTM 8102）作为发酵黑豆的主要菌株。我们研究了LDTM 8102发酵黑豆对太阳紫外线照射（sUV）诱导的皱纹形成的影响。在HaCaT细胞中，LDTM 8102发酵黑豆的乙醇提取物（EFY）有效地减少了sUV诱导的基质金属蛋白酶-1（MMP-1）分泌。EFY的效果优于未发酵（UFY）和乳酸乳球菌KCTC 5854（另一个菌种）发酵的黑豆。此外，EFY降低了sUV诱导的MMP-1 mRNA表达和启动子活性，以及AP-1的反式激活和ERK1/2及JNK1/2的磷酸化。此外，在三维（3D）皮肤培养模型中，EFY减轻了sUV诱导的MMP-1分泌、表皮破坏和胶原蛋白降解。EFY的总多酚含量和抗氧化活性高于UFY。LDTM 8102发酵后，黑豆提取物中有12种代谢物显著（≥2倍）增加。其中，主要异黄酮的代谢物，如6,7,4'-三羟基异黄酮（THIF），发挥了对MMP-1的降低作用，这表明异黄酮代谢物作为活性化合物促成了EFY对MMP-1表达的影响。这些发现表明，EFY是一种有效的天然物质，有可能预防sUV诱导的皱纹形成。
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