Functions of Forkhead Box O on Glucose Metabolism in Abalone and Its Responses to High Levels of Dietary Lipid.

The forkhead box O (FoxO) subfamily is a member of the forkhead transcription factor family. It has regulation functions in glucose metabolism in mammals and fish. In the present study, a gene of the homolog in abalone was cloned. A conservative forkhead (FH) domain and a transactivation (FoxO-TAD) domain were identified. Abalone -specific siRNA (small interfering RNA) was injected to investigate the functions of on glucose metabolism. Knockdown of inhibited expression of phosphoenolpyruvate carboxykinase () and significantly increased expressions of hexokinase () and pyruvate kinase (), but it failed to inhibit the relative mRNA level of glucose-6-phosphatase (). Then, a 100-day feeding trial was conducted to investigate the response of and glucose metabolism in abalone fed with 1.57% (LFD, low-fat diet), 3.82% (MFD, middle-fat diet) and 6.72% (HFD, high-fat diet) of dietary lipid, respectively. The insulin-signaling pathway (AKT) was depressed and FoxO was activated by the HFD, but it did not inhibit glycolysis () or improved gluconeogenesis significantly (). At the same time, impaired hepatopancreas glycogen storage raised hemolymph glucose levels. In conclusion, abalone can be regulated by dietary lipid and can regulate gluconeogenesis or glycolysis in response to changes of dietary lipid levels, in which glycogen metabolism plays an important role.