Dresen Muriel, Schenk Josephine, Berhanu Weldearegay Yenehiwot, Vötsch Désirée, Baumgärtner Wolfgang, Valentin-Weigand Peter, Nerlich Andreas
Institute for Microbiology, Department of Infectious Diseases, University of Veterinary Medicine Hannover, Foundation, 30173 Hannover, Germany.
Institute for Pathology, University of Veterinary Medicine Hannover, Foundation, 30173 Hannover, Germany.
Microorganisms. 2021 Feb 12;9(2):366. doi: 10.3390/microorganisms9020366.
is a common pathogen colonising the respiratory tract of pigs. It can cause meningitis, sepsis and pneumonia leading to economic losses in the pig industry worldwide. Cyclooxygenase-2 (COX-2) and its metabolites play an important regulatory role in different biological processes like inflammation modulation and immune activation. In this report we analysed the induction of COX-2 and the production of its metabolite prostaglandin E (PGE) in a porcine precision-cut lung slice (PCLS) model. Using Western blot analysis, we found a time-dependent induction of COX-2 in the infected tissue resulting in increased PGE levels. Immunohistological analysis revealed a strong COX-2 expression in the proximity of the bronchioles between the ciliated epithelial cells and the adjacent alveolar tissue. The morphology, location and vimentin staining suggested that these cells are subepithelial bronchial fibroblasts. Furthermore, we showed that COX-2 expression as well as PGE production was detected following infection with two prevalent serotypes and that the pore-forming toxin suilysin played an important role in this process. Therefore, this study provides new insights in the response of porcine lung cells to infections and serves as a basis for further studies to define the role of COX-2 and its metabolites in the inflammatory response in porcine lung tissue during infections with .
是一种常见的定植于猪呼吸道的病原体。它可引起脑膜炎、败血症和肺炎，给全球养猪业造成经济损失。环氧合酶-2（COX-2）及其代谢产物在炎症调节和免疫激活等不同生物过程中发挥重要的调节作用。在本报告中，我们在猪精密切割肺片（PCLS）模型中分析了COX-2的诱导及其代谢产物前列腺素E（PGE）的产生。通过蛋白质印迹分析，我们发现在感染组织中COX-2呈时间依赖性诱导，导致PGE水平升高。免疫组织学分析显示，在细支气管附近的纤毛上皮细胞和相邻肺泡组织之间有强烈的COX-2表达。形态学、定位和波形蛋白染色表明这些细胞是支气管上皮下成纤维细胞。此外，我们还表明，在用两种流行血清型感染后检测到了COX-2表达以及PGE产生，并且成孔毒素溶菌酶在这一过程中起重要作用。因此，本研究为猪肺细胞对感染的反应提供了新的见解，并为进一步研究确定COX-2及其代谢产物在感染期间猪肺组织炎症反应中的作用奠定了基础。
