Janet T, Grothe C, Pettmann B, Unsicker K, Sensenbrenner M
Centre de Neurochimie du CNRS, Strasbourg, France.
J Neurosci Res. 1988 Feb;19(2):195-201. doi: 10.1002/jnr.490190204.
The presence of fibroblast growth factor (FGF) was investigated by immunocytochemistry in cultured neuronal cells derived from the peripheral (PNS) and the central nervous system (CNS) of chick and rat embryos. Polyclonal antimouse FGF antibodies, which cross-react with basic and acidic FGF, were used in the peroxidase immunocytochemical staining method. FGF immunoreactivity was found in neurons. Staining intensity in chick and rat brain neuronal cells increased during the culture period, reached a maximum after 6-8 days, and subsequently declined. Embryonic chick ciliary and dorsal root ganglionic (DRG) neurons as well as DRG neurons from newborn rat displayed intense FGF immunoreactivity at 12 and 48 hr in culture. Nonneuronal cells were not stained. The data demonstrate for the first time that cultured neurons from the CNS and PNS contain FGF.
采用免疫细胞化学方法，研究了鸡和大鼠胚胎外周神经系统（PNS）及中枢神经系统（CNS）来源的培养神经元细胞中是否存在成纤维细胞生长因子（FGF）。在过氧化物酶免疫细胞化学染色法中，使用了与碱性和酸性FGF发生交叉反应的多克隆抗小鼠FGF抗体。在神经元中发现了FGF免疫反应性。鸡和大鼠脑神经元细胞的染色强度在培养期间增加，在6 - 8天后达到最大值，随后下降。培养12小时和48小时时，胚胎鸡睫状神经节和背根神经节（DRG）神经元以及新生大鼠的DRG神经元显示出强烈的FGF免疫反应性。非神经元细胞未被染色。这些数据首次证明，来自CNS和PNS的培养神经元含有FGF。
