Wageningen University & Research, Laboratory of Food Chemistry, P.O. Box 17, 6700 AA Wageningen, the Netherlands.
Wageningen University & Research, Laboratory of Food Chemistry, P.O. Box 17, 6700 AA Wageningen, the Netherlands; Unilever Foods Innovation Centre - Hive, Bronland 14, 6708 WH Wageningen, the Netherlands.
Carbohydr Polym. 2021 May 1;259:117781. doi: 10.1016/j.carbpol.2021.117781. Epub 2021 Feb 9.
TEMPO-oxidation of neutral polysaccharides has been used to obtain polyuronides displaying improved functional properties. Although arabinoxylans (AX) from different sources may yield polyuronides with diverse properties due to their variable arabinose (Araf) substitution patterns, information of the TEMPO-oxidation of AX on its structure remains scarce. We oxidized AX using various TEMPO:NaClO:NaOCl ratios. A TEMPO:NaClO:NaOCl ratio of 1.0:2.6:0.4 per mol of Ara gave an oxidized-AX with high molecular weight, minimal effect on xylose appearance, and comprising charged side chains. Although NMR analyses unveiled arabinuronic acid (AraAf) as the only oxidation product in the oxidized-AX, accurate AraA quantification is still challenging. Linkage analysis showed that > 75 % of the β-(1→4)-xylan backbone remained single-substituted at position O-3 of Xyl similarly to native AX. TEMPO-oxidation of AX can be considered a promising approach to obtain arabinuronoxylans with a substitution pattern resembling its parental AX.
TEMPO 氧化中性多糖已被用于获得具有改善功能特性的聚多糖。尽管来自不同来源的阿拉伯木聚糖（AX）由于其不同的阿拉伯糖（Araf）取代模式可能产生具有不同性质的聚多糖，但关于 AX 的 TEMPO 氧化对其结构的信息仍然很少。我们使用不同的 TEMPO:NaClO:NaOCl 比例氧化 AX。TEMPO:NaClO:NaOCl 摩尔比为 1.0:2.6:0.4 对每个 Ara 得到了具有高分子量、最小木糖外观影响和包含带电侧链的氧化-AX。尽管 NMR 分析表明氧化-AX 中的唯一氧化产物是阿拉伯糖醛酸（AraAf），但准确的 AraA 定量仍然具有挑战性。连接分析表明，与天然 AX 一样，β-(1→4)-木聚糖主链中超过 75%的 Xyl 在 O-3 位仍为单取代。AX 的 TEMPO 氧化可以被认为是获得具有与其母体 AX 相似取代模式的阿拉伯糖醛酸木聚糖的有前途的方法。
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