无壳大麦制粉各部分阿拉伯木聚糖的分子特征。
Molecular characterization of arabinoxylans from hull-less barley milling fractions.
机构信息
Grain College, Henan University of Technology, Zhengzhou 450052, Henan Province, China.
出版信息
Molecules. 2011 Mar 24;16(4):2743-53. doi: 10.3390/molecules16042743.
Arabinoxylans were prepared from different hull-less barley milling fractions (bran, shorts and flour). The yields of hull-less bran arabinoxylan (HBB-AX), shorts arabinoxylan (HBS-AX) and flour arabinoxylan (HBF-AX) were 8.42%, 4.08% and 2.13% respectively. Sugar composition analysis showed that arabinose and xylose were the main sugars. HBF-AX had the highest Ara/Xyl ratio, followed by HBS-AX and HBB-AX. Size exclusion chromatography analysis (HPSEC) showed that HBF-AX had the highest molecular weight, followed by HBS-AX and HBB-AX, which had the lowest molecular weight. Aqueous solutions of HBB-AX, HBS-AX and HBF-AX had higher molecular weight(Mw) than in 0.5 mol/L NaOH and 1.0 mol/L NaOH. Dynamic light scattering analysis (DLS) showed that HBB-AX, HBS-AX and HBF-AX had existed in two states in distilled water, 0.5 mol/L NaOH, 1.0 mol/L NaOH, and DMSO/H₂O (90/10), an unaggregated state and an aggregated state, with the latter predominating.
从不同的无壳大麦制粉部分（糠、粗粒和面粉）中制备阿拉伯木聚糖。无壳糠阿拉伯木聚糖（HBB-AX）、粗粒阿拉伯木聚糖（HBS-AX）和面粉阿拉伯木聚糖（HBF-AX）的产率分别为 8.42%、4.08%和 2.13%。糖组成分析表明，阿拉伯糖和木糖是主要的糖。HBF-AX 的 Ara/Xyl 比最高，其次是 HBS-AX 和 HBB-AX。尺寸排阻色谱分析（HPSEC）表明，HBF-AX 的分子量最高，其次是 HBS-AX 和 HBB-AX，它们的分子量最低。HBB-AX、HBS-AX 和 HBF-AX 在水溶液中的分子量（Mw）高于 0.5 mol/L NaOH 和 1.0 mol/L NaOH。动态光散射分析（DLS）表明，HBB-AX、HBS-AX 和 HBF-AX 在蒸馏水、0.5 mol/L NaOH、1.0 mol/L NaOH 和 DMSO/H₂O（90/10）中以两种状态存在，一种是无聚集状态，另一种是聚集状态，后者占主导地位。
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