Department of Chemistry, Graduate School of Science, Tokyo Metropolitan University, Hachioji-shi, Tokyo, Japan.
Department of Viticulture and Enology, University of California, Davis, Davis, CA, USA.
Commun Biol. 2021 Mar 5;4(1):295. doi: 10.1038/s42003-021-01798-8.
Noncoding RNAs (ncRNAs) are involved in various biological processes, including gene expression, development, and disease. Here, we identify a novel consensus sequence of a cis-element involved in long ncRNA (lncRNA) transcription and demonstrate that lncRNA transcription from this cis-element activates meiotic recombination via chromatin remodeling. In the fission yeast fbp1 gene, glucose starvation induces a series of promoter-associated lncRNAs, referred to as metabolic-stress-induced lncRNAs (mlonRNAs), which contribute to chromatin remodeling and fbp1 activation. Translocation of the cis-element required for mlonRNA into a well-characterized meiotic recombination hotspot, ade6-M26, further stimulates transcription and meiotic recombination via local chromatin remodeling. The consensus sequence of this cis-element (mlon-box) overlaps with meiotic recombination sites in the fission yeast genome. At one such site, the SPBC24C6.09c upstream region, meiotic double-strand break (DSB) formation is induced in an mlon-box-dependent manner. Therefore, mlonRNA transcription plays a universal role in chromatin remodeling and the regulation of transcription and recombination.
非编码 RNA(ncRNAs)参与各种生物过程，包括基因表达、发育和疾病。在这里，我们鉴定出一个新的顺式元件的保守序列，该顺式元件参与长 ncRNA(lncRNA)转录，并证明来自该顺式元件的 lncRNA 转录通过染色质重塑激活减数分裂重组。在裂殖酵母 fbp1 基因中，葡萄糖饥饿诱导一系列与启动子相关的 lncRNA，称为代谢应激诱导的 lncRNA(mlonRNA)，有助于染色质重塑和 fbp1 激活。将 mlonRNA 转录所需的顺式元件易位到一个特征明确的减数分裂重组热点 ade6-M26 中，通过局部染色质重塑进一步刺激转录和减数分裂重组。该顺式元件(mlon-box)的保守序列与裂殖酵母基因组中的减数分裂重组位点重叠。在这样的一个位点 SPBC24C6.09c 上游区域，以 mlon-box 依赖的方式诱导减数分裂双链断裂(DSB)的形成。因此，mlonRNA 转录在染色质重塑以及转录和重组的调控中起着普遍的作用。
