Department of Stomatology, Jingzhou Central Hospital, The Second Clinical Medical College, Yangtze University, Jingzhou, China.
Department of Stomatology, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing, China.
Oral Dis. 2022 Sep;28(6):1509-1518. doi: 10.1111/odi.13830. Epub 2021 Mar 19.
To explore the effect of miR-873-5p on proliferation, apoptosis, migration, and invasion of tongue squamous cell carcinoma (TSCC) by targeting SEC11A.
Tongue squamous cell carcinoma tissues were collected and performed by qRT-PCR and Western blotting to determine the expression of miR-873-5p and SPC18. SCC9 and CAL-27 cells were transfected and divided into Mock, mimic NC, miR-873-5p mimic, SEC11A, and miR-873-5p mimic + SEC11A groups. Then, a series of experiments including cell count kit 8 (CCK-8), wound healing, Transwell, and flow cytometry were conducted. Besides, Western blotting was used to detect the expression of SPC18 and EGFR pathway-related proteins.
MiR-873-5p was downregulated while SPC18 was upregulated in TSCC, and miR-873-5p was negatively correlated with SPC18. Dual luciferase reporter gene assay confirmed SEC11A to be a target of miR-873-5p. Cell proliferation, migration, and invasion of SCC9 and CAL-27 cells in miR-873-5p mimic group were decreased with increased cell apoptosis, presenting with downregulations of SPC18 and EGFR pathway-related proteins, while cells in SEC11A group manifested totally different changes. Moreover, the inhibitory effect of miR-873-5p mimic on TSCC cell growth was abolished by SEC11A overexpression.
Overexpression of miR-873-5p may suppress cell proliferation, migration, and invasion, but facilitate apoptosis in TSCC via targeting SEC11A.
通过靶向 SEC11A 探讨 miR-873-5p 对舌鳞癌细胞（TSCC）增殖、凋亡、迁移和侵袭的影响。
收集舌鳞状细胞癌组织，通过 qRT-PCR 和 Western blot 检测 miR-873-5p 和 SPC18 的表达。转染 SCC9 和 CAL-27 细胞并分为 Mock、 mimic NC、miR-873-5p 模拟物、SEC11A 和 miR-873-5p 模拟物+SEC11A 组。然后进行一系列实验，包括细胞计数试剂盒 8（CCK-8）、划痕愈合、Transwell 和流式细胞术。此外，Western blot 用于检测 SPC18 和 EGFR 通路相关蛋白的表达。
在 TSCC 中 miR-873-5p 下调而 SPC18 上调，miR-873-5p 与 SPC18 呈负相关。双荧光素酶报告基因实验证实 SEC11A 是 miR-873-5p 的靶基因。miR-873-5p 模拟物组 SCC9 和 CAL-27 细胞的增殖、迁移和侵袭能力降低，细胞凋亡增加，SPC18 和 EGFR 通路相关蛋白表达下调，而 SEC11A 组细胞则呈现完全相反的变化。此外，SEC11A 过表达可消除 miR-873-5p 模拟物对 TSCC 细胞生长的抑制作用。
过表达 miR-873-5p 可能通过靶向 SEC11A 抑制 TSCC 细胞增殖、迁移和侵袭，促进细胞凋亡。
