Yasuyama T, Inoue K, Kojima T, Sasaki H
Third Department of Internal Medicine, Faculty of Medicine, Toyama Medical and Pharmaceutical University, Japan.
Jpn J Med. 1988 Feb;27(1):34-41. doi: 10.2169/internalmedicine1962.27.34.
In order to elucidate active oxygen in liver diseases, activities, electrophoretic profiles and immunolocalization of superoxide dismutase (SOD) in human liver specimens were investigated. Activities and electrophoresis were studied using liver homogenates in 41 cases and immunolocalization of Cu, Zn SOD was observed in 87 cases. Total SOD activity in acute viral hepatitis (AVH) and fatty liver (FL) groups was significantly lower than that in non-specific reactive hepatitis (NSRH) group. Cu, Zn SOD activity in AVH, FL and chronic active hepatitis (CAH) groups was also significantly lower than that in NSRH group. However, no difference of Mn SOD activity, was found between NSRH group and others. Decreased activity of superoxide dismutase in liver tissues suggests the release of this enzyme from the injured hepatocytes. In electrophoretic patterns of superoxide dismutase, 3 bands of Cu, Zn SOD isozymes and 8 to 10 bands of Mn SOD isozymes were recognized. Immunocytochemical investigation revealed the localization of Cu, Zn SOD in the cytoplasm of hepatocytes. Two different distribution of Cu, Zn SOD was observed in the lobules: a diffuse localization pattern and a focal one. The latter was found in the cases of liver diseases with severe parenchymal lesion. These findings suggest that superoxide radical anion and its scavenger, superoxide dismutase, may play an important role in the pathogenesis of liver cell necrosis.
为了阐明肝脏疾病中的活性氧，我们对人类肝脏标本中超氧化物歧化酶（SOD）的活性、电泳图谱和免疫定位进行了研究。使用41例肝脏匀浆研究了活性和电泳情况，并在87例中观察了铜锌超氧化物歧化酶（Cu, Zn SOD）的免疫定位。急性病毒性肝炎（AVH）组和脂肪肝（FL）组的总超氧化物歧化酶活性显著低于非特异性反应性肝炎（NSRH）组。AVH组、FL组和慢性活动性肝炎（CAH）组的铜锌超氧化物歧化酶活性也显著低于NSRH组。然而，NSRH组与其他组之间未发现锰超氧化物歧化酶（Mn SOD）活性存在差异。肝组织中超氧化物歧化酶活性降低表明该酶从受损肝细胞中释放出来。在超氧化物歧化酶的电泳图谱中，识别出了3条铜锌超氧化物歧化酶同工酶带和8至10条锰超氧化物歧化酶同工酶带。免疫细胞化学研究显示铜锌超氧化物歧化酶定位于肝细胞的细胞质中。在肝小叶中观察到铜锌超氧化物歧化酶有两种不同的分布：弥漫性定位模式和局灶性定位模式。后者见于实质病变严重的肝脏疾病病例。这些发现表明超氧阴离子自由基及其清除剂超氧化物歧化酶可能在肝细胞坏死的发病机制中起重要作用。
