基于高分辨率磁共振成像的幼猪和青少年期家猪（Sus scrofa）图谱。
High-resolution magnetic resonance imaging-based atlases for the young and adolescent domesticated pig (Sus scrofa).
机构信息
Piglet Nutrition & Cognition Laboratory, University of Illinois, Urbana, IL, 61801, USA; Neuroscience Program, University of Illinois, Urbana, IL, 61801, USA.
Neuroscience Program, University of Illinois, Urbana, IL, 61801, USA; Beckman Institute for Advances Science & Technology, University of Illinois, Urbana, IL, 61801, USA.
出版信息
J Neurosci Methods. 2021 Apr 15;354:109107. doi: 10.1016/j.jneumeth.2021.109107. Epub 2021 Mar 3.
BACKGROUND
Neurodevelopmental studies utilize the pig as a translational animal model due to anatomical and morphological similarities between the pig and human brain. However, neuroimaging resources are not as well developed for the pig as they are for humans and other animal models. We established a magnetic resonance imaging-based brain atlas at two different ages for biomedical studies utilizing the pig as a preclinical model.
NEW METHOD
Twenty artificially-reared domesticated male pigs (Sus scrofa) and thirteen sow-reared adolescent domesticated male pigs (Sus scrofa) underwent a series of scans measuring brain macrostructure, microstructure, and arterial cerebral blood volume.
RESULTS
An atlas for the 4-week-old and 12-week-old pig were created along with twenty-six regions of interest. Normative data for brain measures were obtained and detailed descriptions of the data processing pipelines were provided.
COMPARISON WITH EXISTING METHOD
Atlases at the two different ages were created for the pig utilizing newer imaging technology and software. This facilitates the performance of longitudinal studies and enables more precise volume measurements in pigs of various ages by appropriately representing the neuroanatomical features of younger and older pigs and accommodating the proportion differences of the brain over time.
CONCLUSION
Two high-resolution MRI brain atlases specific to the domesticated young and adolescent pig were created using defined image acquisition and data processing methods to facilitate the generation of high-quality normative data for neurodevelopmental research.
背景
由于猪和人类大脑在解剖和形态上的相似性，神经发育研究利用猪作为转化动物模型。然而，与人类和其他动物模型相比，猪的神经影像学资源并不那么发达。我们为利用猪作为临床前模型的生物医学研究建立了基于磁共振成像的大脑图谱，涵盖了两个不同的年龄阶段。
方法
二十只人工饲养的雄性家猪（Sus scrofa）和十三只母猪饲养的青春期雄性家猪（Sus scrofa）接受了一系列扫描，以测量大脑的宏观结构、微观结构和动脉性脑血容量。
结果
为 4 周龄和 12 周龄的猪创建了图谱，共包含 26 个感兴趣区。获得了脑测量的标准数据，并提供了详细的数据处理流程描述。
与现有方法的比较
利用更新的成像技术和软件为猪创建了两个不同年龄的图谱。这便于进行纵向研究，并通过适当表示年幼和年长猪的神经解剖特征以及随着时间的推移适应大脑比例的差异，使不同年龄的猪能够进行更精确的体积测量。
结论
使用定义明确的图像采集和数据处理方法创建了两个针对家养幼猪和青春期猪的高分辨率 MRI 大脑图谱，以方便为神经发育研究生成高质量的标准数据。
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