Programa de Pesquisa Em Biologia Celular e do Desenvolvimento, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro (UFRJ), Cidade Universitária-Ilha do Fundão, Rio de Janeiro, CEP 21941-902, Brazil; Laboratório de Biologia Celular, Departamento de Biologia, Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora (UFJF), Rua José Lourenço Kelmer-Campus, São Pedro, Juiz de Fora, CEP: 36036-900, Brazil.
Programa de Pesquisa Em Biologia Celular e do Desenvolvimento, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro (UFRJ), Cidade Universitária-Ilha do Fundão, Rio de Janeiro, CEP 21941-902, Brazil.
Mol Cell Endocrinol. 2021 May 15;528:111230. doi: 10.1016/j.mce.2021.111230. Epub 2021 Mar 3.
Despite advances in treatment of lethal prostate cancer, the incidence of prostate cancer brain metastases is increasing. In this sense, we analyzed the molecular profile, as well as the functional consequences involved in the reciprocal interactions between prostate tumor cells and human astrocytes. We observed that the DU145 cells, but not the LNCaP cells or the RWPE-1 cells, exhibited more pronounced, malignant and invasive phenotypes along their interactions with astrocytes. Moreover, global gene expression analysis revealed several genes that were differently expressed in our co-culture models with the overexpression of GLIPR1 and SPARC potentially representing a molecular signature associated with the invasion of central nervous system by prostate malignant cells. Further, these results were corroborated by immunohistochemistry and in silico analysis. Thus, we conjecture that the data here presented may increase the knowledge about the molecular mechanisms associated with the invasion of CNS by prostate malignant cells.
尽管在治疗致命性前列腺癌方面取得了进展，但前列腺癌脑转移的发病率仍在增加。有鉴于此，我们分析了分子谱以及前列腺肿瘤细胞与人类星形胶质细胞之间相互作用所涉及的功能后果。我们观察到，DU145 细胞而非 LNCaP 细胞或 RWPE-1 细胞在与星形胶质细胞相互作用时表现出更明显、更恶性和更具侵袭性的表型。此外，全基因表达分析显示，在我们的共培养模型中，几个基因的表达存在差异，其中 GLIPR1 和 SPARC 的过表达可能代表了与前列腺恶性细胞向中枢神经系统侵袭相关的分子特征。此外，这些结果得到了免疫组织化学和计算机分析的证实。因此，我们推测，这里提出的数据可能会增加对与前列腺恶性细胞向中枢神经系统侵袭相关的分子机制的认识。
