Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Department of Medical Laboratory Sciences, Faculty of Medical Sciences, Islamic Azad University, Arak Branch, Arak, Iran.
Biomed Pharmacother. 2021 Jun;138:111391. doi: 10.1016/j.biopha.2021.111391. Epub 2021 Mar 3.
Capparis spinosa (CS) is known as a hypoglycemic medication in many countries. This study was designed to reveal the protective effects of the hydro-ethanolic extract of CS (HECS) fruit against diabetes and oxidative stress in type 2 diabetic rats (T2D). T2D was induced in 4 groups of adult male Sprague Dawley rats, using high fat diet (HFD) and low dose of streptozotocin (STZ). The four groups of diabetic rats were orally gavaged with HECS (200 & 400 mg/kg), metformin (50 mg/kg) or vehicle for 28 days. Two non-diabetic groups were assigned as normal control and HECS treated ones (400 mg/kg). The glucose intolerance, HOMA-IR score, HbA1c level, antioxidative status and expression of genes involved in hepatic gluconeogenesis and lipogenesis were determined. Although HECS had no significant effect on decreasing of HOMA-IR score and HbA1c, it significantly decreased glucose intolerance as well as oxidative stress by reduction of hepatic lipid peroxidation and increase of antioxidant enzymes levels in diabetic rats. Also, HECS treated diabetic rats showed a significant enhanced dyslipidemia, increased weight gain and sera insulin level. In addition, HECS significantly decreased hepatic phosphoenolpyruvate carboxykinase (PEPCK), increased acetyl CoA carboxylase and non-significantly decreased hepatocyte nuclear factor-4α (HNF-4α) as a transactivator of PEPCK at mRNA expression level in diabetic rats. This study indicated the anti-oxidative and anti-diabetic effects of C. spinosa fruit extract and confirmed its traditional usage as a remedy for T2D.
蓟（Capparis spinosa）在许多国家被用作降血糖药物。本研究旨在揭示蓟水-乙醇提取物（HECS）对 2 型糖尿病大鼠（T2D）的糖尿病和氧化应激的保护作用。通过高脂饮食（HFD）和低剂量链脲佐菌素（STZ）诱导 4 组成年雄性 Sprague Dawley 大鼠产生 2 型糖尿病。4 组糖尿病大鼠分别用 HECS（200 和 400mg/kg）、二甲双胍（50mg/kg）或载体灌胃 28 天。另外设立正常对照组和 HECS 处理组（400mg/kg）。测定葡萄糖耐量、HOMA-IR 评分、HbA1c 水平、抗氧化状态以及与肝糖异生和脂生成相关基因的表达。虽然 HECS 对降低 HOMA-IR 评分和 HbA1c 没有显著作用，但它显著降低了糖尿病大鼠的葡萄糖耐量和氧化应激，降低了肝脂质过氧化水平，增加了抗氧化酶水平。此外，HECS 处理的糖尿病大鼠表现出明显的血脂异常、体重增加和血清胰岛素水平升高。此外，HECS 显著降低了糖尿病大鼠肝磷酸烯醇丙酮酸羧激酶（PEPCK）的 mRNA 表达水平，增加了乙酰辅酶 A 羧化酶，非显著降低了作为 PEPCK 转录激活子的肝细胞核因子-4α（HNF-4α）。本研究表明蓟果实提取物具有抗氧化和抗糖尿病作用，证实了其作为 T2D 治疗方法的传统用途。
