Aram J A, Lodge D
Department of Physiology, Royal Veterinary College, London, U.K.
J Neurosci Methods. 1988 Apr;23(3):211-24. doi: 10.1016/0165-0270(88)90005-2.
A simple slice chamber was designed to achieve easy manipulations of temperature, ionic composition and drug concentrations. Spontaneous and evoked extracellular potentials could be recorded with glass microelectrodes from 500 micron thick slices of rat frontal neocortex. In the absence of magnesium ions in the superfusing medium or in the presence of convulsant agents, epileptiform activity was seen. The amplitude of this activity was greatest in layer II/III, each burst consisting of a long-lasting negative potential on the decay phase of which were superimposed many afterpotentials. There were multiple foci from which spontaneous epileptiform bursts spread to other ipsi- and contralateral parts of the cortex via both the grey and white matter. Although such bursts were observed between 23 and 37 degrees C, optimal recording of discrete epileptiform activity was achieved at 29 +/- 1 degrees C. Decreasing extracellular calcium or increasing extracellular concentrations of potassium enhanced burst discharges. Proconvulsant agents initiated both interictal and ictal epileptiform events. This, together with the reduction of epileptiform activity by standard anticonvulsant drugs such as carbamazepine and phenobarbitone suggested that this in vitro model may be useful for studying the pharmacology of epileptogenesis and for developing new therapeutic strategies for epilepsy.
设计了一种简单的脑片槽，以实现对温度、离子成分和药物浓度的轻松操控。使用玻璃微电极可记录来自大鼠额叶新皮质500微米厚脑片的自发和诱发细胞外电位。在灌流介质中不存在镁离子或存在惊厥剂的情况下，可观察到癫痫样活动。这种活动的幅度在第II/III层最大，每次爆发由一个持久的负电位组成，在其衰减阶段叠加有许多后电位。存在多个病灶，自发癫痫样爆发从这些病灶通过灰质和白质扩散到皮质的同侧和对侧其他部位。尽管在23至37摄氏度之间观察到了这种爆发，但在29±1摄氏度时可实现对离散癫痫样活动的最佳记录。降低细胞外钙或增加细胞外钾浓度可增强爆发放电。促惊厥剂引发发作间期和发作期癫痫样事件。这一点，连同卡马西平和苯巴比妥等标准抗惊厥药物对癫痫样活动的抑制作用，表明这种体外模型可能有助于研究癫痫发生的药理学，并为开发新的癫痫治疗策略提供帮助。
