c-Myc G-四链体被荧光探针灵敏且特异性地识别。
c-Myc G-quadruplex is sensitively and specifically recognized by a fluorescent probe.
机构信息
School of Chemistry and Chemical Engineering, Shanghai University of Engineering Science, Shanghai, 201620, PR China; Beijing National Laboratory for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry Chinese Academy of Sciences, Beijing, 100190, PR China.
Beijing National Laboratory for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry Chinese Academy of Sciences, Beijing, 100190, PR China; University of Chinese Academy of Sciences, Beijing, 100049, PR China.
出版信息
Talanta. 2021 May 1;226:122125. doi: 10.1016/j.talanta.2021.122125. Epub 2021 Jan 21.
The G-quadruplex structure formed by the c-myc gene sequence has attracted much attention due to its important physiological function in biology and wide application in nanotechnology. So far, probes capable of recognition of c-myc G-quadruplex with both high specificity and sensitivity are still scarce. This work presented a cyanine dye fluorescent probe named Cy-1, which has almost no fluorescence in aqueous solution, but showing more than 1000-fold fluorescence enhancement for recognizing c-myc G-quadruplex. Cy-1 also has good specificity and can selectively recognize c-myc G-quadruplex from other a variety of G-quadruplex and non-G-quadruplex structures. These properties make Cy-1 a promising probe for c-myc G-quadruplex recognition in nanotechnology or biology.
由于 c-myc 基因序列形成的 G-四链体结构在生物学中具有重要的生理功能，并且在纳米技术中有广泛的应用，因此引起了广泛关注。到目前为止，能够同时具有高特异性和高灵敏度识别 c-myc G-四链体的探针仍然很少。本工作提出了一种名为 Cy-1 的花菁染料荧光探针，它在水溶液中几乎没有荧光，但对识别 c-myc G-四链体的荧光增强超过 1000 倍。Cy-1 还具有良好的特异性，可以选择性地识别 c-myc G-四链体，而不会与其他各种 G-四链体和非 G-四链体结构发生交叉反应。这些性质使 Cy-1 成为纳米技术或生物学中识别 c-myc G-四链体的一种很有前途的探针。
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