Sjögren A, Wang D N, Hovmöller S, Haapasalo M, Ranta H, Kerosuo E, Jousimies-Somer H, Lounatmaa K
Department of Structural Chemistry, Arrhenius Laboratory, University of Stockholm, Sweden.
Mol Microbiol. 1988 Jan;2(1):81-7. doi: 10.1111/j.1365-2958.1988.tb00009.x.
The three-dimensional structures of the crystalline surface layers of two species of Eubacteria have been determined by electron microscopy and computerized image processing. The S-layer of Eubacterium sp. ES4C has tetragonal symmetry, with a unit cell spacing of 10.6 nm and a thickness of 9.5 nm, while that of Eubacterium sp. AHN 990 has hexagonal symmetry a = b = 15.7 nm and a thickness of 13 nm. The resolutions in the reconstructions were 2.5 nm and 1.8 nm, respectively. The reconstruction of the S-layer of strain ES4C reveals a distinct domain structure: a major tetramer, arms connecting adjacent unit cells, and a minor tetramer. The S-layer of strain AHN 990, on the other hand, has a rather complex arrangement, centred around the six-fold axis.
