Laboratorio de Parasitología Molecular, Vicerrectoría de Investigaciones, Universidad El Bosque, Bogotá, Colombia.
Laboratorio de Parasitología Molecular, Vicerrectoría de Investigaciones, Universidad El Bosque, Bogotá, Colombia.
Vet Parasitol Reg Stud Reports. 2021 Jan;23:100520. doi: 10.1016/j.vprsr.2020.100520. Epub 2020 Dec 24.
According to a few parasitological and epidemiological studies, Giardia is the most prevalent parasitic infection among pet dogs in the city of Medellín, the second-largest city in Colombia. This study determined the assemblages of Giardia in the fecal samples of dogs obtained from 18 veterinary centers of Medellín. One hundred fecal samples of dogs diagnosed with Giardia using microscopy were analyzed via nested polymerase chain reaction (PCR) using three genes (gdh, bg, and tpi). The PCR products were purified and sequenced, and phylogenetic analyses were conducted using the maximum likelihood algorithm of the three loci. From the 100 samples analyzed, 47 were Giardia-positive via PCR. Genotypes C and D were detected in six samples, neither of which were associated with human infection. However, the zoonotic potential of Giardia cannot be ruled out because of the small number of samples that could be sequenced for assemblage assignation.
根据一些寄生虫学和流行病学研究，贾第虫是哥伦比亚第二大城市麦德林市宠物狗中最普遍的寄生虫感染。本研究确定了从麦德林市 18 个兽医中心获得的狗粪便样本中的贾第虫组合。使用显微镜诊断患有贾第虫的 100 份狗粪便样本通过巢式聚合酶链反应 (PCR) 用三个基因 (gdh、bg 和 tpi) 进行分析。PCR 产物经过纯化和测序，并使用三个基因座的最大似然算法进行系统发育分析。在分析的 100 个样本中，有 47 个通过 PCR 呈贾第虫阳性。在 6 个样本中检测到基因型 C 和 D，它们都与人类感染无关。然而，由于可用于确定组合的样本数量较少，因此不能排除贾第虫的人畜共患潜力。
