依托咪酯对 reuniens 神经元活动的影响。
Effect of etomidate on the reuniens neuronal activity.
机构信息
Department of Anesthesiology, First Affiliated Hospitalof Wannan Medical College, Wuhu Anhui 241000, China.
出版信息
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2021 Jan 28;46(1):39-46. doi: 10.11817/j.issn.1672-7347.2021.190576.
OBJECTIVES
To explore the effect of etomidate on the neuronal activity of ventral thalamic reuniens nucleus and the underlying mechanisms.
METHODS
Whole-cell patch clamp method was used to explore the effect of etomidate on the activity of ventral thalamic reuniens neurons in the acute brain slices obtained from 4-5 weeks old C57BL/6J mice. The electrophysiological characteristics of ventral thalamic reuniens neurons were recorded in the current clamp mode, and then the effects of etomidate (0.5, 2.0, 8.0 μmol/L etomidate groups) and intralipid (intralipid group) on the discharge frequency and membrane potential of ventral thalamic reuniens neurons were recorded. During the experiment, the ventral thalamic reuniens neuron firing rates (RNFRs) were recorded as F, F and F before, after administration, and after elution; and the membrane potential was recorded as MP and MP before, after administration. The chlorine channel of gamma-amino butyric acid Type A (GABAA) receptor was blocked with 100 μmol/L picrotoxin (PTX). The RNFRs were recorded as F, F and F before, after perfusing etomidate with sub-anesthesia concentration (0.5 μmol/L) and after perfusing both PTX and etomidate.
RESULTS
In the intralipid group, there was no significant difference among the F, F and F (>0.05). But in the etomidate groups (0.5, 2.0, 8.0 μmol/L), the F was less than the F, there was significant difference (all <0.01); the F was higher than the F, there was significant difference (all <0.05). Moreover, there was significant difference in the inhibitory degree of the RNFRs between the 0.5 μmol/L etomidate group and the 8.0 μmol/L etomidate group (<0.05). In the experiment to explore the mechanism of etomidate (0.5 μmol/L), the F was compared with the F, there was significant difference (<0.01); but when the F was compared with the F, there was no significant difference (>0.05).
CONCLUSIONS
Etomidate can inhibit the activity of ventral thalamic reuniens neurons in concentration-dependent manner, and which is reversible. Etomidate with sub-anesthetic concentration inhibits the activity of ventral thalamic reuniens neurons via targeting the GABA receptor.
目的
探讨依托咪酯对腹侧丘脑联合核神经元活动的影响及其机制。
方法
采用全细胞膜片钳技术，在 4-5 周龄 C57BL/6J 小鼠急性脑片中观察依托咪酯对腹侧丘脑联合核神经元活动的影响。在电流钳模式下记录腹侧丘脑联合核神经元的电生理特性，然后记录依托咪酯（0.5、2.0、8.0μmol/L 依托咪酯组）和脂肪乳（脂肪乳组）对腹侧丘脑联合核神经元放电频率和膜电位的影响。实验过程中，以 F、F 和 F 记录腹侧丘脑联合核神经元放电率（RNFRs），分别为给药前、给药后和洗脱后；以 MP 和 MP 记录膜电位，分别为给药前和给药后。用 100μmol/L 荷包牡丹碱（PTX）阻断γ-氨基丁酸 A 型（GABAA）受体氯通道。在亚麻醉浓度（0.5μmol/L）下用依托咪酯灌流后，以 F、F 和 F 记录 RNFRs，在 PTX 和依托咪酯灌流后以 F、F 和 F 记录 RNFRs。
结果
在脂肪乳组中，F、F 和 F 之间差异无统计学意义（>0.05）。但在依托咪酯组（0.5、2.0、8.0μmol/L）中，F 小于 F，差异有统计学意义（均<0.01）；F 大于 F，差异有统计学意义（均<0.05）。此外，0.5μmol/L 依托咪酯组与 8.0μmol/L 依托咪酯组的 RNFRs 抑制程度差异有统计学意义（<0.05）。在探讨依托咪酯（0.5μmol/L）作用机制的实验中，F 与 F 相比，差异有统计学意义（<0.01）；但 F 与 F 相比，差异无统计学意义（>0.05）。
结论
依托咪酯呈浓度依赖性抑制腹侧丘脑联合核神经元的活动，且具有可逆性。亚麻醉浓度的依托咪酯通过作用于 GABA 受体抑制腹侧丘脑联合核神经元的活动。
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