Zhu Jun, Hao Suyu, Zhang Xinyue, Qiu Jingyue, Xuan Qin, Ye Liping
Administrative Office, Shanghai Basilica Clinic, Shanghai, China.
Shuangwu Information Technical Company Ltd., Shanghai, China.
Front Genet. 2021 Feb 18;11:637320. doi: 10.3389/fgene.2020.637320. eCollection 2020.
Malignant melanoma represents a sort of neoplasm deriving from melanocytes or cells developing from melanocytes. The balance of energy and energy-associated body composition and body mass index could be altered by exercise, thereby directly affecting the microenvironment of neoplasm. However, few studies have examined the mechanism of genes induced by exercise and the pathways involved in melanoma. This study used three separate datasets to perform comprehensive bioinformatics analysis and then screened the probable genes and pathways in the process of exercise-promoted melanoma. In total, 1,627 differentially expressed genes (DEGs) induced by exercise were recognized. All selected genes were largely enriched in NF-kappa B, Chemokine signaling pathways, and the immune response after gene set enrichment analysis. The protein-protein interaction network was applied to excavate DEGs and identified the most relevant and pivotal genes. The top 6 hub genes (Itgb2, Wdfy4, Itgam, Cybb, Mmp2, and Parp14) were identified, and importantly, 5 hub genes (Itgb2, Wdfy4, Itgam, Cybb, and Parp14) were related to weak disease-free survival and overall survival (OS). In conclusion, our findings demonstrate the prognostic value of exercise-induced genes and uncovered the pathways of these genes in melanoma, implying that these genes might act as prognostic biomarkers for melanoma.
恶性黑色素瘤是一种起源于黑素细胞或由黑素细胞发育而来的细胞的肿瘤。运动可改变能量及与能量相关的身体成分和体重指数的平衡，从而直接影响肿瘤的微环境。然而，很少有研究探讨运动诱导基因的机制以及黑色素瘤所涉及的途径。本研究使用三个独立的数据集进行全面的生物信息学分析，然后筛选运动促进黑色素瘤过程中可能的基因和途径。总共识别出1627个由运动诱导的差异表达基因（DEG）。在基因集富集分析后，所有选定的基因主要富集于核因子κB、趋化因子信号通路和免疫反应。应用蛋白质-蛋白质相互作用网络挖掘DEG，并确定最相关和关键的基因。确定了前6个枢纽基因（整合素β2、含WD40结构域的蛋白4、整合素αM、细胞色素b2、基质金属蛋白酶2和聚（ADP-核糖）聚合酶14），重要的是，5个枢纽基因（整合素β2、含WD40结构域的蛋白4、整合素αM、细胞色素b2和聚（ADP-核糖）聚合酶14）与无病生存期和总生存期较差相关。总之，我们的研究结果证明了运动诱导基因的预后价值，并揭示了这些基因在黑色素瘤中的途径，这意味着这些基因可能作为黑色素瘤的预后生物标志物。
