Cross A J, Slater P, Skan W
Department of Physiology, University of Manchester.
Neuropeptides. 1988 Feb-Mar;11(2):73-6. doi: 10.1016/0143-4179(88)90013-3.
The high affinity binding of 125I-Bolton-Hunter labelled CCK-8 (125I-BHCCK) was studied in human brain using ligand binding and autoradiographic techniques. High levels of 125I-BHCCK binding were observed in cortex, striatum and cerebellum. In cerebellar membranes 125I-BHCCK binding was inhibited by CCK analogues with an order of potency, caerulein greater than CCK-8 greater than CCK-33 greater than gastrin 1-17 = CCK-8 non-sulphated. CCK-4 was inactive. It is suggested that in human brain 125I-BHCCK labels a population of CCK receptors similar to those observed in guinea pig brain. The properties and distribution of these receptors differ from those of rat brain.
利用配体结合和放射自显影技术，在人脑组织中研究了125I-博尔顿-亨特标记的胆囊收缩素-8（125I-BHCCK）的高亲和力结合情况。在大脑皮层、纹状体和小脑中观察到高水平的125I-BHCCK结合。在小脑膜中，CCK类似物对125I-BHCCK结合的抑制作用具有一定的效力顺序，即蛙皮素大于CCK-8大于CCK-33大于胃泌素1-17 = 非硫酸化CCK-8。CCK-4无活性。研究表明，在人脑中，125I-BHCCK标记的CCK受体群体与在豚鼠脑中观察到的相似。这些受体的特性和分布与大鼠脑不同。
