全自动免疫组化检测法检测福尔马林固定石蜡包埋肺肿瘤组织中的 Napsin-A

Automated Immunohistochemistry Assay for the Detection of Napsin-A in Formalin-Fixed Paraffin-Embedded Tissues from Lung Tumors.

机构信息

Southern Pathology Services Inc., Ponce, Puerto Rico.

Pathology Division, Ponce Health Sciences University, Ponce, Puerto Rico.

出版信息

Methods Mol Biol. 2021;2279:23-33. doi: 10.1007/978-1-0716-1278-1_3.

DOI:10.1007/978-1-0716-1278-1_3

PMID:33683683

Abstract

Immunohistochemistry (IHC) enables the selective detection of proteins in cells of formalin-fixed-paraffin-embedded (FFPE) tissue sections. This technique plays a key role in the identification and classification of primary lung cancer tumors through the evaluation of the expression of the aspartic proteinase Napsin-A. However, immunohistochemistry is a complex process involving many critical steps and the lack of standardization as well as inappropriate analytical conditions may contribute to inconsistent results between laboratories. Automated immunohistochemistry addresses this issue by ensuring the quality and the reproducibility of the results among different laboratories. Here we describe an automated IHC protocol used in our laboratory for the detection of Napsin-A in FFPE lung tissue sections.

摘要

免疫组织化学（IHC）能够选择性地检测福尔马林固定石蜡包埋（FFPE）组织切片中的蛋白质。该技术通过评估天冬氨酸蛋白酶 Napsin-A 的表达，在原发性肺癌肿瘤的鉴定和分类中发挥着关键作用。然而，免疫组织化学是一个复杂的过程，涉及许多关键步骤，缺乏标准化以及不适当的分析条件可能导致实验室之间的结果不一致。自动化免疫组织化学通过确保不同实验室之间结果的质量和可重复性来解决这个问题。在这里，我们描述了我们实验室中用于检测 FFPE 肺组织切片中天冬氨酸蛋白酶 Napsin-A 的自动化免疫组织化学方案。