Aix Marseille Univ, APHM, INSERM, MMG, Hôpital la Timone, Service de Biologie Cellulaire, Marseille, France.
Methods Mol Biol. 2021;2279:157-164. doi: 10.1007/978-1-0716-1278-1_12.
The detection of molecular alterations such as ROS1 and ALK rearrangements is performed as part of the diagnosis of advanced-stage lung adenocarcinoma. These alterations allow the treatments with tyrosine kinase inhibitors. Cytological samples are very useful as up to 40% patients are diagnosed with this type of sample. Here we describe the immunocytochemistry technique usable to reveal the overexpression of ALK or ROS1 tyrosine kinase receptors secondary to ALK and ROS1 rearrangements, respectively.
ROS1 和 ALK 重排等分子改变的检测是晚期肺腺癌诊断的一部分。这些改变可以用酪氨酸激酶抑制剂进行治疗。细胞学样本非常有用，因为高达 40%的患者通过这种类型的样本进行诊断。在这里，我们描述了免疫细胞化学技术，该技术可用于揭示 ALK 和 ROS1 重排分别导致的 ALK 和 ROS1 酪氨酸激酶受体的过表达。
