Aix Marseille University, APHM, INSERM, MMG, la Timone Hospital, Cell Biology Laboratory, 13005 Marseille, France.
Aix Marseille University, APHM, CHU Nord, Oncobiology Laboratory, 13015 Marseille, France.
Cells. 2023 Feb 27;12(5):754. doi: 10.3390/cells12050754.
Lung cancer has become the leading cause of cancer death for men and women. Most patients are diagnosed at an advanced stage when surgery is no longer a therapeutic option. At this stage, cytological samples are often the less invasive source for diagnosis and the determination of predictive markers. We assessed the ability of cytological samples to perform diagnosis, and to establish molecular profile and PD-L1 expression, which are essential for the therapeutic management of patients.
We included 259 cytological samples with suspected tumor cells and assessed the ability to confirm the type of malignancy by immunocytochemistry. We summarized results of molecular testing by next generation sequencing (NGS) and PD-L1 expression from these samples. Finally, we analyzed the impact of these results in the patient management.
Among the 259 cytological samples, 189 concerned lung cancers. Of these, immunocytochemistry confirmed the diagnosis in 95%. Molecular testing by NGS was obtained in 93% of lung adenocarcinomas and non-small cell lung cancer. PD-L1 results were obtained in 75% of patients tested. The results obtained with cytological samples led to a therapeutic decision in 87% of patients.
Cytological samples are obtained by minimally invasive procedures and can provide enough material for the diagnosis and therapeutic management in lung cancer patients.
肺癌已成为男性和女性癌症死亡的主要原因。大多数患者在诊断时已处于晚期，此时手术已不再是一种治疗选择。在这个阶段，细胞学样本通常是诊断和确定预测标志物的侵袭性较小的来源。我们评估了细胞学样本在诊断、建立分子谱和 PD-L1 表达方面的能力，这些对于患者的治疗管理至关重要。
我们纳入了 259 份疑似肿瘤细胞的细胞学样本，并评估了免疫细胞化学确认恶性肿瘤类型的能力。我们总结了这些样本中通过下一代测序（NGS）进行的分子检测和 PD-L1 表达的结果。最后，我们分析了这些结果对患者管理的影响。
在 259 份细胞学样本中，有 189 份涉及肺癌。其中，免疫细胞化学诊断在 95%的病例中得到了证实。93%的肺腺癌和非小细胞肺癌进行了 NGS 分子检测。对 75%的患者进行了 PD-L1 检测。细胞学样本的检测结果在 87%的患者中导致了治疗决策。
细胞学样本通过微创程序获得，可以为肺癌患者的诊断和治疗管理提供足够的材料。
