Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, SP, Brazil.
Núcleo de Pesquisas em Ciências Biológicas & Departamento de Ciências Biológicas, Instituto de Ciências Exatas e Biológicas, Universidade Federal de Ouro Preto, Ouro Preto, MG, Brazil.
Methods Mol Biol. 2021;2279:225-239. doi: 10.1007/978-1-0716-1278-1_18.
Aldehydes are abundantly present in tobacco smoke and in urban air pollution and are endogenously generated as products of the lipid peroxidation process. These molecules can react with DNA bases forming mutagenic exocyclic adducts, which have been used as biomarkers of aldehyde exposure and as potential tools for the study of inflammation, metal storage diseases, neurodegenerative disorders, and cancer. High-performance liquid chromatography-tandem mass spectrometry (HPLC/MS/MS) provides a highly precise, specific and ultrasensitive method for the detection of exocyclic DNA adducts. Here we present and describe a validated micro-HPLC-Electro Spray Ionization (ESI)-MS/MS method for the quantification of 1,N-propanodGuo, an adduct produced following the reaction between 2'-deoxyguanosine and acetaldehyde or crotonaldehyde.
醛类物质大量存在于烟草烟雾和城市空气污染中，并且作为脂质过氧化过程的产物而内源性生成。这些分子可以与 DNA 碱基反应形成诱变的环外加合物，这些加合物已被用作醛暴露的生物标志物，并作为研究炎症、金属储存疾病、神经退行性疾病和癌症的潜在工具。高效液相色谱-串联质谱（HPLC/MS/MS）为检测环外 DNA 加合物提供了一种高度精确、特异和灵敏的方法。在这里，我们提出并描述了一种经过验证的微高效液相色谱-电喷雾电离（ESI）-MS/MS 方法，用于定量分析 1，N-丙二酰基鸟嘌呤，这是脱氧鸟苷与乙醛或巴豆醛反应生成的加合物。
