Biochemistry and Biophysics Center, National Heart Lung and Blood Institute, National Institutes of Health, Bethesda, MD, 20892, USA.
Curr Opin Cell Biol. 2021 Aug;71:21-28. doi: 10.1016/j.ceb.2021.01.013. Epub 2021 Mar 5.
Protein coats, important for vesicular trafficking in eukaryotic cells, help shape membranes and package cargo. But their dynamic construction cannot be fully understood until the distinct steps of their assembly in their native intracellular context at molecular resolution can be visualized. For this, correlative light and electron microscopy (CLEM) is an essential tool. Here, we discuss how emerging CLEM techniques have been used to study the assembly of protein coats inside cells. We review how current and developing CLEM technologies are poised to answer fundamental questions of protein coat architecture at the nanoscale.
蛋白质外壳对于真核细胞的囊泡运输非常重要,它们帮助塑造膜并包装货物。但是,直到能够以分子分辨率在其天然的细胞内环境中可视化其组装的各个独特步骤,才能充分理解它们的动态构建。为此,相关的光镜和电子显微镜(CLEM)是必不可少的工具。在这里,我们讨论了新兴的 CLEM 技术如何用于研究细胞内蛋白质外壳的组装。我们回顾了当前和正在开发的 CLEM 技术如何解决纳米尺度上蛋白质外壳结构的基本问题。