Janicki P K, Siembab D, Paulo E A, Krzaścik P
Department of Pharmacology, Medical Academy of Warsaw, Poland.
Pharmacology. 1988;36(3):183-7. doi: 10.1159/000138382.
Nifedipine concentrations in plasma and brain tissue of rats were assayed from 5 min to 4 h after an intravenous injection of 6 mg/kg body weight. Samples of rat plasma and homogenized brain tissue were extracted under sodium light with a dichloromethane-pentane mixture. Analyses were done by high-performance liquid chromatography on a reverse-phase C18 column with electrochemical detection. The peak concentration of nifedipine in brain obtained after 10 min was 37 micrograms/g wet weight tissue, the half-life was 75 min. Apparently, nifedipine can easily cross the blood-brain barrier in the rat.
在给大鼠静脉注射6毫克/千克体重后5分钟至4小时，测定大鼠血浆和脑组织中的硝苯地平浓度。大鼠血浆和匀浆脑组织样本在钠灯下用二氯甲烷 - 戊烷混合物进行萃取。采用高效液相色谱法在反相C18柱上进行分析，并用电化学检测。10分钟后在脑中获得的硝苯地平峰值浓度为37微克/克湿重组织，半衰期为75分钟。显然，硝苯地平在大鼠中能够轻易穿过血脑屏障。
