Clinical Research Fellow, Academic Department of Trauma and Orthopaedics, School of Medicine, University of Leeds, Clarendon Wing, Level A, Great George Street, Leeds, LS1 3EX, West Yorkshire,
Eur Cell Mater. 2021 Mar 9;41:269-315. doi: 10.22203/eCM.v041a19.
Platelet products (PP) and bone-marrow aspirate are popular sources of osteoinductive signalling molecules and osteogenic bone marrow mesenchymal stromal cells (BM-MSCs) used in the treatment of impaired bone healing. However, the combined use of PP and BM-MSCs in clinical studies has reported mixed results. Understanding the cellular and molecular interactions between PP and BM-MSCs plays the important role of guiding future research and clinical application. This systematic review investigates the effects of PP on the biophysiological functions of BM-MSCs in in vitro human studies, including (i) proliferation, (ii) migration, (iii) differentiation, (iv) growth factor/cytokine/protein expression, (v) immunomodulation, (vi) chemotactic effect on haematopoietic stem cells, (vii) response to apoptotic stress, and (viii) gene expression. In vitro studies in human have demonstrated the multi-faceted 'priming effect' of PP on the biophysiological functions of BM-MSCs. PP has been shown to improve proliferation, migration, osteogenic differentiation, reaction to apoptotic stress as well as immunomodulatory, pro-angiogenic and pro-inflammatory capacities of BM-MSCs. Several factors are highlighted that restrict the transferability of these findings into clinical practice. Therefore, more collaborative in vitro research in humans modelled to reflect clinical practice is required to better understand the effects of PP exposure on the biophysiological function(s) of BM-MSCs in human.
血小板制品 (PP) 和骨髓抽吸物是骨诱导信号分子和用于治疗骨愈合受损的成骨骨髓间充质基质细胞 (BM-MSCs) 的常用来源。然而，在临床研究中联合使用 PP 和 BM-MSCs 的报告结果喜忧参半。了解 PP 与 BM-MSCs 之间的细胞和分子相互作用对于指导未来的研究和临床应用具有重要作用。本系统评价研究了 PP 在体外人类研究中对 BM-MSCs 生物生理功能的影响，包括：(i) 增殖、(ii) 迁移、(iii) 分化、(iv) 生长因子/细胞因子/蛋白表达、(v) 免疫调节、(vi) 对造血干细胞的趋化作用、(vii) 对凋亡应激的反应以及 (viii) 基因表达。体外人类研究表明，PP 对 BM-MSCs 的生物生理功能具有多方面的“启动效应”。PP 已被证明可提高 BM-MSCs 的增殖、迁移、成骨分化、对凋亡应激的反应以及免疫调节、促血管生成和促炎能力。有几个因素被强调，这些因素限制了将这些发现转化为临床实践的可转移性。因此，需要更多针对反映临床实践的人类的合作性体外研究，以更好地了解 PP 暴露对 BM-MSCs 生物生理功能的影响。
