Production and characteristics of elemental sulfur during simultaneous nitrate and sulfide removal.

The production and characteristics of elemental sulfur were examined during simultaneous sulfide and nitrate removal, with abiotic assays as control. The biotic assay showed good sulfide and nitrate removal, with the respective removal percentage of which were 90.67-96.88% and 100%. Nitrate reduction resulted in the production of nitrogen gas, while sulfate formed due to sulfide oxidation. The concentration of elemental sulfur in the effluent was greater than that in the sludge, which accounted for 73.70-86.28% of total elemental sulfur produced. Furthermore, the elemental sulfur of the effluent and sludge from the biotic assays was orthorhombic crystal S. Elemental sulfur was normally distributed in the effluent, but its average diameter increased with the increasing influent sulfide concentration (60-300 mg S/L), where the average diameter increased from 10 (60 mg S/L) to 29 μm (300 mg S/L).