体外研究胶质母细胞瘤干细胞样细胞放射敏感性的方法。
In Vitro Methods for the Study of Glioblastoma Stem-Like Cell Radiosensitivity.
机构信息
Radiation Oncology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.
Department of Clinical Neurosciences, University of Cambridge, Cambridge, UK.
出版信息
Methods Mol Biol. 2021;2269:37-47. doi: 10.1007/978-1-0716-1225-5_3.
Ionizing radiation is a critical component of glioblastoma (GBM) therapy. Recent data have implicated glioblastoma stem-like cells (GSCs) as determinants of GBM development, maintenance, and treatment response. Understanding the response of GSCs to radiation should thus provide insight into the development of improved GBM treatment strategies. Towards this end, in vitro techniques for the analysis of GSC radiosensitivity are an essential starting point. One such method, the clonogenic survival assay has been adapted to assessing the intrinsic radiosensitivity of GSCs and is described here. As an alternative method, the limiting dilution assay is presented for defining the radiosensitivity of GSC lines that do not form colonies or only grow as neurospheres. In addition to these cellular strategies, we describe γH2AX foci analysis, which provides a surrogate marker for radiosensitivity at the molecular level. Taken together, the in vitro methods presented here provide tools for defining intrinsic radiosensitivity of GSCs and for testing agents that may enhance GBM radioresponse.
电离辐射是胶质母细胞瘤（GBM）治疗的一个关键组成部分。最近的数据表明，胶质母细胞瘤干细胞样细胞（GSCs）是 GBM 发展、维持和治疗反应的决定因素。因此，了解 GSCs 对辐射的反应应该为开发改进的 GBM 治疗策略提供深入的了解。为此，分析 GSC 放射敏感性的体外技术是一个重要的起点。克隆形成存活测定法是一种评估 GSCs 内在放射敏感性的方法，在此进行描述。作为替代方法，提出了限制稀释测定法来定义不形成集落或仅作为神经球生长的 GSC 系的放射敏感性。除了这些细胞策略外，我们还描述了γH2AX 焦点分析，它为分子水平的放射敏感性提供了替代标记。总之，这里介绍的体外方法为确定 GSCs 的内在放射敏感性以及测试可能增强 GBM 放射反应的药物提供了工具。
Zotero 插件