Department of Cranio Maxillofacial Surgery, University of Leipzig Medical Center, Leipzig, Germany.
Department of Hematology, Cell Therapy and Hemostaseology, University of Leipzig Medical Center, Leipzig, Germany.
Methods Mol Biol. 2021;2269:175-201. doi: 10.1007/978-1-0716-1225-5_13.
Bench-to-bedside axis of therapeutic product development is currently being oriented towards minimum invasiveness on both ends-not only clinical application but harvesting of the starting biological material as well. This is particularly relevant for Advanced Therapy Medicinal Products and their specific legislative requirements, even more so in skin regeneration. It is precisely the skin equivalents and grafts that benefit from the minimum-to-noninvasive approach to a noteworthy extent, taking in account the sensitive nature of both skin harvesting and grafting.This chapter includes protocols for two separate steps of generating skin equivalent from the cells cultured from hair follicle outer root sheath. The first step is a non-pigmented epidermal equivalent generated from human keratinocytes from the outer root sheath named non-pigmented epidermal graft. The second step consists of co-cultivating human keratinocytes and human melanocytes from the outer root sheath, hereby producing a pigmented epidermal graft.
治疗产品开发的从基础到临床的转化目前正朝着两端的微创化方向发展——不仅是临床应用，还有起始生物材料的采集。这对于先进治疗药物产品及其特定的立法要求尤其相关，在皮肤再生方面更是如此。正是皮肤等效物和移植物从很大程度上受益于这种微创到非侵入性的方法，同时考虑到皮肤采集和移植的敏感性。本章包括从毛囊外根鞘培养的细胞生成皮肤等效物的两个独立步骤的方案。第一步是从外根鞘产生的非色素化表皮等效物，称为非色素化表皮移植物。第二步包括共培养来自外根鞘的人角质形成细胞和人黑素细胞，从而产生色素化表皮移植物。
