从多能干细胞生成神经干细胞以鉴定早期神经元发育。
Generation of Neural Stem Cells from Pluripotent Stem Cells for Characterization of Early Neuronal Development.
机构信息
Martin Luther University Halle-Wittenberg, University Clinic and Outpatient Clinic for Psychiatry, Psychotherapy, and Psychosomatic Medicine, Halle/Saale, Germany.
出版信息
Methods Mol Biol. 2021;2269:233-244. doi: 10.1007/978-1-0716-1225-5_16.
PMID:33687683
Abstract
We describe the protocol for the efficient in vitro differentiation of human neural stem cells (NSCs) from human-induced pluripotent stem cells (iPS cells). NSCs differentiate via neural epithelial progenitors enabling the analysis of early neuronal development. They represent neural progenitor cells, which are capable of differentiating into neurons and glia.
摘要
我们介绍了一种从人诱导多能干细胞（iPS 细胞）高效体外分化为人类神经干细胞（NSC）的方案。NSC 通过神经上皮祖细胞分化，从而能够分析早期神经元发育。它们代表神经祖细胞，能够分化为神经元和神经胶质细胞。
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