UCL Centre for Nerve Engineering, Department of Pharmacology, UCL School of Pharmacy, University College London, London, UK.
Methods Mol Biol. 2021;2269:245-254. doi: 10.1007/978-1-0716-1225-5_17.
Peripheral nerves have a limited ability to regenerate and current clinical approaches involving microsurgery give suboptimal recovery. Engineered tissues using aligned cellular collagen hydrogels can be used as in vitro models through the incorporation of human Schwann cells. However, primary human Schwann cells are difficult to obtain and can be challenging to culture. The ability to generate Schwann cells from human-induced pluripotent stem cells (hiPSCs) provides a more reliable cell source for modeling peripheral nerve tissue. Here, we describe protocols for generating hiPSC-derived Schwann cells and incorporating them into 3D engineered tissue culture models for peripheral nerve research.
周围神经的再生能力有限，目前涉及显微外科的临床方法恢复效果不佳。使用定向细胞胶原水凝胶的工程组织可通过纳入人雪旺细胞作为体外模型。然而，原代人雪旺细胞难以获得，培养也具有挑战性。从人诱导多能干细胞（hiPSC）生成雪旺细胞的能力为周围神经组织建模提供了更可靠的细胞来源。在此，我们描述了生成 hiPSC 衍生雪旺细胞的方案，并将其纳入用于周围神经研究的 3D 工程组织培养模型。
