Chang Wenlong, Zhao Jing, Liu Lu, Xing Xiaoming, Zhang Chao, Meng Huihong, Gopinath Subash C B, Liu Yonggang
Endocrine Laboratory, Baoding No.1 Central Hospital, Baoding, Hebei 071000, China.
Department of First Neurology, Baoding No.1 Central Hospital, Baoding, Hebei 071000, China.
J Anal Methods Chem. 2021 Feb 22;2021:6661799. doi: 10.1155/2021/6661799. eCollection 2021.
Nanotechnology is playing a major role in the field of medical diagnosis, in particular with the biosensor and bioimaging. It improves the performance of the desired system dramatically by displaying higher selectivity and sensitivity. Carbon nanomaterial, gold nanostructure, magnetite nanoparticle, and silica substrate are the most popular nanomaterials greatly contributed to make the affordable and effective biosensor at low-cost. This research work is introducing a new sensing strategy with graphene oxide-constructed triangular electrodes to diagnose Alzheimer's disease (AD). MicroRNA-137 (miRNA-137) was found as a suitable biomarker for AD, and the sensing method was established here to detect miRNA-137 on the complementary sequence. To enhance the immobilization of capture miRNA-137, gold nanostar (GNS) was conjugated with capture miRNA and immobilized on the GO-modified surface through an amine linker. This immobilization process enhanced the hybridization of the target and reaches the detection limit at 10 fM with the sensitivity of 1 fM on the linear curve with a regression coefficient of 0.9038. Further control sequences of miRNA-21 and single and triple base mismatched miRNA-137 did not show a significant response in current changes, indicating the specific miRNA-137 detection for diagnosing AD.
纳米技术在医学诊断领域发挥着重要作用，特别是在生物传感器和生物成像方面。它通过展现出更高的选择性和灵敏度，极大地提升了所需系统的性能。碳纳米材料、金纳米结构、磁铁矿纳米颗粒和二氧化硅基底是最受欢迎的纳米材料，它们为低成本制造经济实惠且有效的生物传感器做出了巨大贡献。这项研究工作正在引入一种采用氧化石墨烯构建的三角形电极来诊断阿尔茨海默病（AD）的新传感策略。发现微小RNA - 137（miRNA - 137）是AD的合适生物标志物，并在此建立了在互补序列上检测miRNA - 137的传感方法。为了增强捕获miRNA - 137的固定化，将金纳米星（GNS）与捕获miRNA缀合，并通过胺连接子固定在GO修饰的表面上。这种固定化过程增强了靶标的杂交，在10 fM时达到检测限，线性曲线上灵敏度为1 fM，回归系数为0.9038。miRNA - 21的进一步对照序列以及单碱基和三碱基错配的miRNA - 137在电流变化中未显示出显著响应，表明该方法对诊断AD具有miRNA - 137特异性检测能力。
