College of Pharmaceutical Sciences, Medical Research Institute, Southwest University, Chongqing 400715, China.
Org Lett. 2021 Mar 19;23(6):2337-2341. doi: 10.1021/acs.orglett.1c00458. Epub 2021 Mar 10.
A fungal C-alkylated dihydroisocoumarin pathway was discovered and elucidated. This pathway includes the following (1) a nonreducing polyketide synthase and a P450 collaboratively synthesize hydroxylated C-methylated isocoumarin ; (2) a methyltransferase methylates to ; and (3) importantly, an esterase specifically catalyzes a ring reconstruction process of to C-alkylated dihydroisocoumarin . Our discovery fills the gap in the enzymatic transformation process of natural C-alkylated isocoumarin derivates.
发现并阐明了一种真菌 C-烷基二氢异香豆素途径。该途径包括以下步骤：(1) 一个非还原型聚酮合酶和一个 P450 共同合成羟基化的 C-甲基异香豆素；(2) 一个甲基转移酶将 甲基化；(3) 重要的是，一个酯酶特异性地催化 到 C-烷基二氢异香豆素的环重建过程。我们的发现填补了天然 C-烷基异香豆素衍生物酶转化过程中的空白。
