Department of Pharmacology and Toxicology, School of Pharmacy, Hamadan University of Medical Sciences, Hamadan, Iran.
Department of Physiology, School of Medicine, Neurophysiology Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.
J Ethnopharmacol. 2021 Jun 12;273:113990. doi: 10.1016/j.jep.2021.113990. Epub 2021 Mar 6.
In Iranian folkloric medicine, Bupleurum falcatum L. (Chinese Thoroughwax) has been used as a selective analgesic remedy for several centuries.
The current research was conducted to explore the anti-nociceptive and anti-allodynic action of Bupleurum falcatum L. roots essential oil (BFEO) in Swiss mice.
Formalin-induced paw licking (FIPL) model was applied for exploring of BFEO antinociceptive effects (neurogenic or inflammatory pain). The involvements of L-arginine-NO-cGMP-KATP channel pathway and several receptors such as opioid, peroxisome proliferator-activated (PPA), cannabinoid, transient receptor potential vanilloid, and adrenergic receptors were assesses to detect the anti-nociceptive activity of BFEO. Cervical spinal cord contusion (CSC) paradigm was employed for induction of neuropathic pain.
BFEO (100 mg/kg), in the FIPL model, produced significant antinociception compared to the control mice (p < 0.01). Furthermore, L-arginine, methylene blue, glibenclamide, naloxonazine, GW9662, and SR141716A pre-treatments restored the BFEO anti-nociceptive effects (p < 0.05) in the FIPL (second phase) test (p < 0.05). Intraperitoneal administration of saikosaponin A (one of the main constituents of BFEO) partially alleviated (p < 0.05) pain in FIPL test. Likewise, in CSC mice, the von Frey assay exhibited that BFEO could alter mechanical allodynia.
Finally, it seems that, in male mice, BFEO has both anti-allodynic and anti-nociceptive effects. The present data also suggest activating the L-arginine-NO-cGMP-KATP channel pathway as well as interaction of opioid, PPA, and cannabinoid receptors in the BFEO anti-nociceptive activities. These results also propose that BFEO could effectively attenuate allodynia in CSC mice.
在伊朗民间医学中，柴胡（Chinese Thoroughwax）已被用作数百年的选择性镇痛药。
本研究旨在探索柴胡根精油（BFEO）在瑞士小鼠中的抗伤害感受和抗异常性疼痛作用。
应用福尔马林诱导的爪舔（FIPL）模型探索 BFEO 的镇痛作用（神经源性或炎症性疼痛）。评估 L-精氨酸-NO-cGMP-KATP 通道途径和几种受体（如阿片、过氧化物酶体增殖物激活、大麻素、瞬时受体电位香草素和肾上腺素能受体）的参与，以检测 BFEO 的镇痛活性。颈脊髓挫伤（CSC）范式用于诱导神经性疼痛。
BFEO（100mg/kg）在 FIPL 模型中与对照组小鼠相比产生显著的镇痛作用（p<0.01）。此外，L-精氨酸、亚甲蓝、格列本脲、naloxonazine、GW9662 和 SR141716A 预处理恢复了 BFEO 的抗伤害感受作用（p<0.05）在 FIPL（第二阶段）测试（p<0.05）。腹腔内给予柴胡皂苷 A（BFEO 的主要成分之一）部分缓解了 FIPL 测试中的疼痛（p<0.05）。同样，在 CSC 小鼠中，von Frey 测定表明 BFEO 可以改变机械性异常性疼痛。
最后，似乎 BFEO 对雄性小鼠具有抗异常性疼痛和抗伤害感受作用。本研究数据还表明，BFEO 的抗伤害感受作用涉及 L-精氨酸-NO-cGMP-KATP 通道途径的激活以及阿片、过氧化物酶体增殖物激活和大麻素受体的相互作用。这些结果还表明 BFEO 可以有效减轻 CSC 小鼠的异常性疼痛。
